ASGPR1/Asialoglycoprotein Receptor 1/ASGR1 Antibody (8D7): sc-52623

ASGPR1 Antibody (8D7) is a mouse monoclonal IgG1 kappa light chain antibody that detects ASGPR1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). ASGPR1 (8D7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The Asialoglycoprotein Receptor (ASGPR), also known as hepatic lectin, is a type II integral membrane protein predominantly expressed in hepatic cells, where ASGPR plays a crucial role in glycoprotein clearance from circulation. ASGPR1 is composed of two homologous subunits, ASGPR1 and ASGPR2, which form multimeric complexes and contain four functional domains: a cytosolic domain, a transmembrane domain, a stalk domain, and a carbohydrate recognition domain (CRD). The CRD enables ASGPR to specifically bind glycoproteins with terminal galactose and N-acetylgalactosamine residues in calcium′s presence, facilitating ASGPR-glycoprotein complex internalization into cells. Upon internalization, receptor and ligand dissociate, allowing ASGPR to recycle back to cell membrane. ASGPR can interact with hepatitis B virus (HBV), mediating HBV entry into liver cells, highlighting ASGPR′s potential as a therapeutic target in HBV infection.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

ASGPR1/Asialoglycoprotein Receptor 1/ASGR1 Antibody (8D7) References:

1. Crystal structure of the carbohydrate recognition domain of the H1 subunit of the asialoglycoprotein receptor.  |  Meier, M., et al. 2000. J Mol Biol. 300: 857-65. PMID: 10891274
2. Detection of surface asialoglycoprotein receptor expression in hepatic and extra-hepatic cells using a novel monoclonal antibody.  |  Park, JH., et al. 2006. Biotechnol Lett. 28: 1061-9. PMID: 16799763
3. [Optimized expression of a single-chain Fv antibody against human asialoglycoprotein receptor and determination of its affinity constant].  |  Wang, WY., et al. 2006. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 22: 504-6. PMID: 16806017
4. Inhibition of hepatocarcinoma by systemic delivery of Apoptin gene via the hepatic asialoglycoprotein receptor.  |  Peng, DJ., et al. 2007. Cancer Gene Ther. 14: 66-73. PMID: 16874360
5. Asialoglycoprotein receptor mediated hepatocyte targeting - strategies and applications.  |  D'Souza, AA. and Devarajan, PV. 2015. J Control Release. 203: 126-39. PMID: 25701309
6. Autoantibodies to asialoglycoprotein receptor (ASGPR) in patients with autoimmune liver diseases.  |  Villalta, D., et al. 2015. Clin Chim Acta. 450: 1-5. PMID: 26220739
7. LYTACs that engage the asialoglycoprotein receptor for targeted protein degradation.  |  Ahn, G., et al. 2021. Nat Chem Biol. 17: 937-946. PMID: 33767387
8. High-yield purification and characterization of human asialoglycoprotein receptor.  |  Treichel, U., et al. 1995. Protein Expr Purif. 6: 251-5. PMID: 7663158
9. The major subunit of the asialoglycoprotein receptor is expressed on the hepatocellular surface in mice lacking the minor receptor subunit.  |  Braun, JR., et al. 1996. J Biol Chem. 271: 21160-6. PMID: 8702886
10. Receptor-mediated entry of hepatitis B virus particles into liver cells.  |  Treichel, U., et al. 1997. Arch Virol. 142: 493-8. PMID: 9349295
11. Detection of the asialoglycoprotein receptor on cell lines of extrahepatic origin.  |  Park, JH., et al. 1998. Biochem Biophys Res Commun. 244: 304-11. PMID: 9514919
12. Preliminary clinical study of the distribution of HPMA copolymers bearing doxorubicin and galactosamine.  |  Julyan, PJ., et al. 1999. J Control Release. 57: 281-90. PMID: 9895415

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ASGPR1/Asialoglycoprotein Receptor 1/ASGR1 Antibody (8D7) has 15 citations in a variety of scientific publications.

Primary antibodies like Anti-ASGPR1/Asialoglycoprotein Receptor 1/ASGR1 Antibody (8D7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ASGPR1/Asialoglycoprotein Receptor 1/ASGR1 Antibody (8D7) Specifications

Alternate Product Name: asialoglycoprotein (ASGPR) receptor 1

Product Clone Name: 8D7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: ASGPR1

Epitope Species: rat

Product Reactivity/Detection: ASGPR1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 15 citations in various scientific publications

Available Conjugates/Forms for ASGPR1/Asialoglycoprotein Receptor 1/ASGR1 Antibody (8D7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, B: Biotin, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Asialoglycoprotein Receptor 1/2/ASGR1/ASGR2 antibody is Asialoglycoprotein Receptor 1/2/ASGR1/ASGR2 Antibody (E-1): sc-166633.