ASC/TMS1/PYCARD Antibody (F-9): sc-271054

ASC Antibody (F-9) is a mouse monoclonal IgG1 kappa light chain antibody that targets the apoptosis-associated speck-like protein containing a CARD (ASC), also known as TMS1 or PYCARD. Anti-ASC antibody (F-9) is raised against the N-terminal amino acids 1-120 of human ASC and is effective in detecting ASC proteins from mouse, rat, and human sources using techniques such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). ASC is a critical member of the CARD-containing adaptor protein family, playing a pivotal role in apoptotic signaling by mediating interactions between adaptor proteins like Apaf-1 and pro-caspases such as CASP9. Structurally, ASC comprises a Pyrin-like domain (PYD) at the N-terminus and an 87-residue caspase recruitment domain (CARD) at the C-terminus, which are essential for its function in apoptosis regulation. Upon induction of apoptosis, ASC undergoes a transformation from diffuse cytoplasmic distribution to forming punctate or spherical perinuclear aggregates, a process that can be visualized through fluorescence microscopy. This aggregation is significant in the cellular response to apoptotic stimuli, including treatments with retinoic acid and various anti-tumor drugs. Additionally, ASC expression has been observed in multiple leukemia and melanoma cell lines, indicating potential as a biomarker in cancer research. Available in both non-conjugated and various conjugated forms, including horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® variants, ASC (F-9) antibody serves as a versatile and essential tool for researchers studying apoptotic mechanisms and related cellular pathways.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

ASC/TMS1/PYCARD Antibody (F-9) References:

1. ASC, a novel 22-kDa protein, aggregates during apoptosis of human promyelocytic leukemia HL-60 cells.  |  Masumoto, J., et al. 1999. J Biol Chem. 274: 33835-8. PMID: 10567338
2. TMS1, a novel proapoptotic caspase recruitment domain protein, is a target of methylation-induced gene silencing in human breast cancers.  |  Conway, KE., et al. 2000. Cancer Res. 60: 6236-42. PMID: 11103776
3. Activation of a caspase-9-mediated apoptotic pathway by subcellular redistribution of the novel caspase recruitment domain protein TMS1.  |  McConnell, BB. and Vertino, PM. 2000. Cancer Res. 60: 6243-7. PMID: 11103777
4. The pyrin domain: a possible member of the death domain-fold family implicated in apoptosis and inflammation.  |  Martinon, F., et al. 2001. Curr Biol. 11: R118-20. PMID: 11250163
5. ASC speck formation as a readout for inflammasome activation.  |  Stutz, A., et al. 2013. Methods Mol Biol. 1040: 91-101. PMID: 23852599
6. Assembly and regulation of ASC specks.  |  Hoss, F., et al. 2017. Cell Mol Life Sci. 74: 1211-1229. PMID: 27761594
7. Comprehensive review of ASC structure and function in immune homeostasis and disease.  |  Agrawal, I. and Jha, S. 2020. Mol Biol Rep. 47: 3077-3096. PMID: 32124174
8. Crosstalk Between the NLRP3 Inflammasome/ASC Speck and Amyloid Protein Aggregates Drives Disease Progression in Alzheimer's and Parkinson's Disease.  |  Hulse, J. and Bhaskar, K. 2022. Front Mol Neurosci. 15: 805169. PMID: 35185469
9. The gray zone squamous lesions: Asc-US / Asc-H.  |  Kattoor, J. and Kamal, MM. 2022. Cytojournal. 19: 30. PMID: 35673698
10. ASC Regulates Subcutaneous Adipose Tissue Lipogenesis and Lipolysis via p53/AMPKα Axis.  |  Chen, H., et al. 2022. Int J Mol Sci. 23: PMID: 36077447
11. ASC specks exacerbate α‑synuclein pathology via amplifying NLRP3 inflammasome activities.  |  Zheng, R., et al. 2023. J Neuroinflammation. 20: 26. PMID: 36740674
12. Nanoscale organization of the endogenous ASC speck.  |  Glück, IM., et al. 2023. iScience. 26: 108382. PMID: 38047065

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ASC/TMS1/PYCARD Antibody (F-9) has 115 citations in a variety of scientific publications.

Primary antibodies like Anti-ASC/TMS1/PYCARD Antibody (F-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ASC/TMS1/PYCARD Antibody (F-9) Specifications

Alternate Product Name: TMS1

Product Clone Name: F-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-120

Epitope Target: ASC

Epitope Species: human

Product Reactivity/Detection: ASC

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 115 citations in various scientific publications

Available Conjugates/Forms for ASC/TMS1/PYCARD Antibody (F-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin