ASB-6 Antibody (H-8): sc-515649
- ASB-6 Antibody (H-8) is a mouse monoclonal IgG1 κ ASB-6 antibody, cited in 2 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 245-269 within an internal region of ASB-6 of human origin
- ASB-6 Antibody (H-8) is recommended for detection of ASB-6 of human origin by WB, IP, IF and ELISA
- Anti-ASB-6 Antibody (H-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ASB-6 Antibody (H-8) for WB applications. These reagents are now offered in bundles with ASB-6 Antibody (H-8) (see ordering information below).
ASB-6 Antibody (H-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects ASB-6 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ASB-6 (H-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ASB-6, or ankyrin repeat and SOCS box-containing 6, is a 421 amino acid protein that plays a crucial role in the ubiquitin-proteasome pathway, functioning as a substrate-recognition component of an E3 ubiquitin-protein ligase complex. ASB-6 is primarily localized in the cytoplasm, where ASB-6 collaborates with other proteins to facilitate the ubiquitination and subsequent proteasomal degradation of target proteins, thereby regulating various cellular processes, including signal transduction and protein turnover. The presence of the SOCS box domain in ASB-6 is particularly important as this domain allows for interaction with the Elongin B/C complex, which is essential for the ubiquitination process. Understanding ASB-6 function and significance helps researchers gain insights into ASB-6′s role in cellular homeostasis and potential implications in diseases where protein degradation pathways are disrupted.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ASB-6 Antibody (H-8) References:
- The SOCS box: a tale of destruction and degradation. | Kile, BT., et al. 2002. Trends Biochem Sci. 27: 235-41. PMID: 12076535
- Molecular cloning and characterization of the human ASB-8 gene encoding a novel member of ankyrin repeat and SOCS box containing protein family. | Liu, Y., et al. 2003. Biochem Biophys Res Commun. 300: 972-9. PMID: 12559969
- Asb6, an adipocyte-specific ankyrin and SOCS box protein, interacts with APS to enable recruitment of elongins B and C to the insulin receptor signaling complex. | Wilcox, A., et al. 2004. J Biol Chem. 279: 38881-8. PMID: 15231829
- Identification and characterization of ankyrin repeat and SOCS box-containing gene ASB15 in silico. | Yoshida, K. 2005. Int J Mol Med. 16: 343-7. PMID: 16012773
- ASB proteins interact with Cullin5 and Rbx2 to form E3 ubiquitin ligase complexes. | Kohroki, J., et al. 2005. FEBS Lett. 579: 6796-802. PMID: 16325183
- Ankyrin repeat and suppressors of cytokine signaling box protein asb-9 targets creatine kinase B for degradation. | Debrincat, MA., et al. 2007. J Biol Chem. 282: 4728-4737. PMID: 17148442
- Characterization of porcine ASB6 gene and transcripts-comparison of mammalian transcripts. | Robic, A., et al. 2008. Anim Biotechnol. 19: 138-43. PMID: 18607786
- Ankyrin repeat and suppressor of cytokine signaling (SOCS) box-containing protein (ASB) 15 alters differentiation of mouse C2C12 myoblasts and phosphorylation of mitogen-activated protein kinase and Akt. | McDaneld, TG. and Spurlock, DM. 2008. J Anim Sci. 86: 2897-902. PMID: 18641171
- Asb6 upregulation by Areca nut extracts is associated with betel quid-induced oral carcinogenesis. | Hung, KF., et al. 2009. Oral Oncol. 45: 543-8. PMID: 19251471
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ASB-6 Antibody (H-8) | sc-515649 | 200 µg/ml | $322.00 | |||
ASB-6 Antibody (H-8): m-IgG Fc BP-HRP Bundle | sc-531455 | 200 µg Ab; 10 µg BP | $361.00 | |||
ASB-6 Antibody (H-8): m-IgGκ BP-HRP Bundle | sc-525147 | 200 µg Ab, 40 µg BP | $361.00 | |||
ASB-6 Antibody (H-8): m-IgG1 BP-HRP Bundle | sc-544567 | 200 µg Ab; 20 µg BP | $361.00 | |||
ASB-6 Antibody (H-8) AC | sc-515649 AC | 500 µg/ml, 25% agarose | $424.00 | |||
ASB-6 Antibody (H-8) HRP | sc-515649 HRP | 200 µg/ml | $322.00 | |||
ASB-6 Antibody (H-8) FITC | sc-515649 FITC | 200 µg/ml | $336.00 | |||
ASB-6 Antibody (H-8) PE | sc-515649 PE | 200 µg/ml | $349.00 | |||
ASB-6 Antibody (H-8) Alexa Fluor® 488 | sc-515649 AF488 | 200 µg/ml | $364.00 | |||
ASB-6 Antibody (H-8) Alexa Fluor® 546 | sc-515649 AF546 | 200 µg/ml | $364.00 | |||
ASB-6 Antibody (H-8) Alexa Fluor® 594 | sc-515649 AF594 | 200 µg/ml | $364.00 | |||
ASB-6 Antibody (H-8) Alexa Fluor® 647 | sc-515649 AF647 | 200 µg/ml | $364.00 | |||
ASB-6 Antibody (H-8) Alexa Fluor® 680 | sc-515649 AF680 | 200 µg/ml | $364.00 | |||
ASB-6 Antibody (H-8) Alexa Fluor® 790 | sc-515649 AF790 | 200 µg/ml | $364.00 | |||
ASB-6 (H-8) Neutralizing Peptide | sc-515649 P | 100 µg/0.5 ml | $69.00 |