ARSA Antibody (H-7): sc-390568
- ARSA Antibody (H-7) is a mouse monoclonal IgG1 κ ARSA antibody provided at 200 µg/ml
- raised against amino acids 1-161 mapping at the N-terminus of ARSA of human origin
- ARSA Antibody (H-7) is recommended for detection of ARSA of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
- Anti-ARSA Antibody (H-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ARSA Antibody (H-7) for WB applications. These reagents are now offered in bundles with ARSA Antibody (H-7) (see ordering information below).
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ARSA Antibody (H-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects ARSA protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ARSA (H-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ARSA protein plays a crucial role in cellular detoxification processes, as ARSA is involved in the hydrolysis of ATP in the presence of anionic substrates such as antimonite, which is essential for providing resistance to toxic compounds like arsenite and antimonite. This function is vital for maintaining cellular homeostasis and protecting cells from the harmful effects of heavy metals. The active form of ARSA exists as a homodimer featuring four nucleotide binding sites, two from each monomer, which is significant for enzymatic activity and interaction with substrates. Understanding ARSA function and interactions can provide insights into mechanisms of resistance to environmental toxins and potential therapeutic targets for related diseases.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ARSA Antibody (H-7) References:
- The ATPase mechanism of ArsA, the catalytic subunit of the arsenite pump. | Walmsley, AR., et al. 1999. J Biol Chem. 274: 16153-61. PMID: 10347168
- Mechanism of the ArsA ATPase. | Rosen, BP., et al. 1999. Biochim Biophys Acta. 1461: 207-15. PMID: 10581357
- Conformational changes in four regions of the Escherichia coli ArsA ATPase link ATP hydrolysis to ion translocation. | Zhou, T., et al. 2001. J Biol Chem. 276: 30414-22. PMID: 11395509
- Antimonite regulation of the ATPase activity of ArsA, the catalytic subunit of the arsenical pump. | Walmsley, AR., et al. 2001. Biochem J. 360: 589-97. PMID: 11736648
- Unisite and multisite catalysis in the ArsA ATPase. | Zhou, T., et al. 2002. J Biol Chem. 277: 23815-20. PMID: 11964412
- Biochemical evidence for interaction between the two nucleotide binding domains of ArsA. Insights from mutants and ATP analogs. | Jia, H. and Kaur, P. 2003. J Biol Chem. 278: 6603-9. PMID: 12488319
- Nonequivalence of the nucleotide binding domains of the ArsA ATPase. | Jiang, Y., et al. 2005. J Biol Chem. 280: 9921-6. PMID: 15637064
- Molecular characterization of an anion pump. The ArsB protein is the membrane anchor for the ArsA protein. | Tisa, LS. and Rosen, BP. 1990. J Biol Chem. 265: 190-4. PMID: 1688427
- The plasmid-encoded arsenical resistance pump: an anion-translocating ATPase. | Rosen, BP. 1990. Res Microbiol. 141: 336-41. PMID: 1704144
- Molecular analysis of an ATP-dependent anion pump. | Rosen, BP., et al. 1990. Philos Trans R Soc Lond B Biol Sci. 326: 455-63. PMID: 1970649
- Identification of a Novel ARSA Gene Mutation Through High-Throughput Molecular Diagnosis Method in Two Girls with Late Infantile Metachromatic Leukodystrophy. | Yari, A., et al. 2023. Neuromolecular Med. 25: 563-572. PMID: 37682448
- Interaction of ATP binding sites in the ArsA ATPase, the catalytic subunit of the Ars pump. | Li, J., et al. 1996. J Biol Chem. 271: 25247-52. PMID: 8810286
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ARSA Antibody (H-7) | sc-390568 | 200 µg/ml | $322.00 | |||
ARSA Antibody (H-7): m-IgG Fc BP-HRP Bundle | sc-530192 | 200 µg Ab; 10 µg BP | $361.00 | |||
ARSA Antibody (H-7): m-IgGκ BP-HRP Bundle | sc-523586 | 200 µg Ab, 40 µg BP | $361.00 | |||
ARSA Antibody (H-7): m-IgG1 BP-HRP Bundle | sc-543918 | 200 µg Ab; 20 µg BP | $361.00 | |||
ARSA Antibody (H-7) AC | sc-390568 AC | 500 µg/ml, 25% agarose | $424.00 | |||
ARSA Antibody (H-7) HRP | sc-390568 HRP | 200 µg/ml | $322.00 | |||
ARSA Antibody (H-7) FITC | sc-390568 FITC | 200 µg/ml | $336.00 | |||
ARSA Antibody (H-7) PE | sc-390568 PE | 200 µg/ml | $349.00 | |||
ARSA Antibody (H-7) Alexa Fluor® 488 | sc-390568 AF488 | 200 µg/ml | $364.00 | |||
ARSA Antibody (H-7) Alexa Fluor® 546 | sc-390568 AF546 | 200 µg/ml | $364.00 | |||
ARSA Antibody (H-7) Alexa Fluor® 594 | sc-390568 AF594 | 200 µg/ml | $364.00 | |||
ARSA Antibody (H-7) Alexa Fluor® 647 | sc-390568 AF647 | 200 µg/ml | $364.00 | |||
ARSA Antibody (H-7) Alexa Fluor® 680 | sc-390568 AF680 | 200 µg/ml | $364.00 | |||
ARSA Antibody (H-7) Alexa Fluor® 790 | sc-390568 AF790 | 200 µg/ml | $364.00 |