Arp3 Antibody (B-1): sc-374200

Arp3 Antibody (B-1) is a mouse monoclonal IgG2b light chain antibody that detects Arp3 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Arp3 is a crucial component of the Arp2/3 complex, which plays a vital role in actin polymerization, a process essential for various cellular functions including cell migration, adhesion, and the formation of cellular protrusions. The Arp2/3 complex facilitates the nucleation of new actin filaments, enabling the rapid reorganization of the cytoskeleton in response to extracellular signals. This dynamic restructuring is particularly important in processes such as chemotaxis, where cells must move toward chemical signals, and in the formation of lamellipodia and filopodia, which are critical for cell movement and shape changes. The interaction of Arp3 with other proteins, such as the WASP family, is essential for the activation of the Arp2/3 complex, highlighting Arp3′s role in linking signaling pathways to cytoskeletal dynamics. Anti-Arp3 antibody (B-1) enables researchers to gain insights into the mechanisms of actin regulation and its implications in various physiological and pathological processes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Arp3 Antibody (B-1) References:

1. Actin -related protein 3 (Arp3) is mutated in proteinuric BUF/Mna rats.  |  Akiyama, K., et al. 2008. Mamm Genome. 19: 41-50. PMID: 18064521
2. Nucleotide-mediated conformational changes of monomeric actin and Arp3 studied by molecular dynamics simulations.  |  Dalhaimer, P., et al. 2008. J Mol Biol. 376: 166-83. PMID: 18155236
3. Tobacco Arp3 is localized to actin-nucleating sites in vivo.  |  Maisch, J., et al. 2009. J Exp Bot. 60: 603-14. PMID: 19129161
4. Regulation of spermiogenesis, spermiation and blood-testis barrier dynamics: novel insights from studies on Eps8 and Arp3.  |  Cheng, CY. and Mruk, DD. 2011. Biochem J. 435: 553-62. PMID: 21486226
5. ARP3 promotes tumor metastasis and predicts a poor prognosis in hepatocellular carcinoma.  |  Lv, J., et al. 2018. Pathol Res Pract. 214: 1356-1361. PMID: 30049513
6. Rac1/Wave2/Arp3 Pathway Mediates Rat Blood-Brain Barrier Dysfunction under Simulated Microgravity Based on Proteomics Strategy.  |  Yan, R., et al. 2021. Int J Mol Sci. 22: PMID: 34068233
7. mTORC1/rpS6 and mTORC2/PKC regulate spermatogenesis through Arp3-mediated actin microfilament organization in Eriocheir sinensis.  |  Li, ZF., et al. 2023. Cell Tissue Res. 393: 559-575. PMID: 37328709
8. Cryo-EM structures reveal how phosphate release from Arp3 weakens actin filament branches formed by Arp2/3 complex.  |  Chavali, SS., et al. 2024. Nat Commun. 15: 2059. PMID: 38448439
9. Dragon's blood attenuates LPS-induced intestinal epithelial barrier dysfunction via upregulation of FAK-DOCK180-Rac1-WAVE2-Arp3 and downregulation of TLR4/NF-κB signaling pathways.  |  Liu, H., et al. 2024. J Nat Med. 78: 1013-1028. PMID: 39014275
10. The Schizosaccharomyces pombe actin-related protein, Arp3, is a component of the cortical actin cytoskeleton and interacts with profilin.  |  McCollum, D., et al. 1996. EMBO J. 15: 6438-46. PMID: 8978671

The preferred Arp3 antibody is Arp3 Antibody (A-1): sc-48344.