Arnt 2 CRISPR/Cas9 KO Plasmid (h): sc-403101
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Arnt 2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Arnt 2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Arnt 2 Antibody (B-11): sc-393683
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Arnt 2 CRISPR/Cas9 KO Plasmid (h) | sc-403101 | 20 µg | $397.00 |
Overview
ARNT2 (aryl hydrocarbon receptor nuclear translocator 2) encodes a bHLH-PAS transcription factor that heterodimerizes with partners such as HIF family members to regulate stimulus-responsive gene expression programs. Arnt 2 contributes to oxygen-sensing and developmental transcriptional networks by supporting DNA binding at hypoxia response elements and coordinating chromatin-dependent control of target loci. It is highly relevant to studies of neuronal differentiation and cellular adaptation to metabolic or environmental stress, where ARNT2-dependent transcription can influence survival and lineage-specific gene signatures. Dysregulation of ARNT2-associated pathways has been linked to altered hypoxic signaling outputs and neurodevelopmental phenotypes, making it a useful node for mechanistic interrogation of PAS-domain transcriptional circuitry.
Arnt 2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ARNT2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ARNT2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ARNT2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Arnt 2 protein expression.
This CRISPR knockout system enables efficient generation of ARNT2-deficient cell models for investigation of Arnt 2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ARNT2 exon(s) critical for Arnt 2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ARNT2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Arnt 2 CRISPR/Cas9 KO Plasmid (h) and Arnt 2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ARNT2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Arnt 2 HDR Plasmid (h) and Arnt 2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ARNT2 homology arms to support homology-directed repair at defined ARNT2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.