ARMCX1 CRISPR/Cas9 KO Plasmid (h): sc-407214
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ARMCX1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ARMCX1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ARMCX1 Antibody (G-5): sc-376291
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ARMCX1 CRISPR/Cas9 KO Plasmid (h) | sc-407214 | 20 µg | $397.00 |
Overview
ARMCX1 (armadillo repeat containing, X-linked 1) encodes a mitochondria-associated armadillo-repeat protein implicated in coordinating mitochondrial dynamics, trafficking, and quality control in mammalian cells. Through interactions that influence mitochondrial transport and fusion–fission balance, ARMCX1 can shape cellular bioenergetics, redox homeostasis, and apoptotic susceptibility, linking it to stress-response programs. In neuronal and other polarized cell types, ARMCX1-related mechanisms are often studied in the context of organelle distribution along cytoskeletal networks and their impact on cell survival. Altered ARMCX1 expression has been reported across cancer and neurobiology datasets, motivating mechanistic research on how ARMCX1-dependent mitochondrial regulation contributes to disease-associated phenotypes.
ARMCX1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ARMCX1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ARMCX1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ARMCX1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ARMCX1 protein expression.
This CRISPR knockout system enables efficient generation of ARMCX1-deficient cell models for investigation of ARMCX1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ARMCX1 exon(s) critical for ARMCX1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ARMCX1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ARMCX1 CRISPR/Cas9 KO Plasmid (h) and ARMCX1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ARMCX1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ARMCX1 HDR Plasmid (h) and ARMCX1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ARMCX1 homology arms to support homology-directed repair at defined ARMCX1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.