Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ARK-2 CRISPR/Cas9 KO Plasmid (m): sc-423197
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ARK-2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ARK-2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ARK-2 Antibody (A-3): sc-393357
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ARK-2 CRISPR/Cas9 KO Plasmid (m) | sc-423197 | 20 µg | $397.00 |
Overview
Aurkb (ARK-2) encodes a serine/threonine kinase that functions as the catalytic core of the chromosome passenger complex, coordinating chromosome condensation, kinetochore–microtubule attachment error correction, and cytokinesis. ARK-2 activity is regulated across mitosis through phosphorylation-dependent signaling and spatial relocalization from centromeres to the spindle midzone, coupling spindle assembly checkpoint satisfaction to faithful chromosome segregation. Disruption of Aurkb perturbs chromatin dynamics and contractile ring formation, leading to aneuploidy and micronuclei, processes frequently leveraged to study genome instability. Altered AURKB signaling has been implicated in proliferative phenotypes and tumor-associated chromosomal instability, making it a common node in cell cycle and mitotic fidelity research.
ARK-2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Aurkb gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Aurkb together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Aurkb open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ARK-2 protein expression.
This CRISPR knockout system enables efficient generation of Aurkb-deficient cell models for investigation of ARK-2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Aurkb exon(s) critical for ARK-2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Aurkb genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ARK-2 CRISPR/Cas9 KO Plasmid (m) and ARK-2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Aurkb locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ARK-2 HDR Plasmid (m) and ARK-2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Aurkb homology arms to support homology-directed repair at defined Aurkb target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.