Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ARK-1 CRISPR/Cas9 KO Plasmid (m): sc-423198
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ARK-1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ARK-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ARK-1 Antibody (35C1): sc-56881
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ARK-1 CRISPR/Cas9 KO Plasmid (m) | sc-423198 | 20 µg | $397.00 |
Overview
Aurka encodes the serine/threonine kinase ARK-1 (Aurora kinase A), a core regulator of centrosome maturation, mitotic entry, and bipolar spindle assembly in mouse cells. ARK-1 coordinates phosphorylation events that support microtubule dynamics and chromosome segregation, functioning within cell-cycle control networks that include PLK1- and CDK1-dependent mitotic progression and checkpoint surveillance. Dysregulated Aurka activity perturbs genomic stability and has been associated with abnormal proliferation phenotypes relevant to oncogenic transformation and aneuploidy. As a result, Aurka is widely used as a mechanistic node to study mitotic fidelity, spindle assembly defects, and cell-cycle–linked stress responses.
ARK-1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Aurka gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Aurka together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Aurka open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ARK-1 protein expression.
This CRISPR knockout system enables efficient generation of Aurka-deficient cell models for investigation of ARK-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Aurka exon(s) critical for ARK-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Aurka genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ARK-1 CRISPR/Cas9 KO Plasmid (m) and ARK-1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Aurka locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ARK-1 HDR Plasmid (m) and ARK-1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Aurka homology arms to support homology-directed repair at defined Aurka target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.