ARH Antibody (E-10): sc-514263

ARH Antibody (E-10) is a mouse monoclonal IgG2a antibody that detects ARH (autosomal recessive hypercholesterolemia protein) in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ARH is a 308 amino acid cytoplasmic protein that plays a crucial role in the endocytosis of the low-density lipoprotein receptor (LDLR) by stabilizing its interaction with the structural components of coated pits in polarized cells, particularly in the liver and lymphocytes. This function is vital for maintaining cholesterol homeostasis, as defects in the ARH gene lead to autosomal recessive hypercholesterolemia, a condition characterized by impaired internalization of LDL receptors, resulting in significantly elevated plasma LDL cholesterol levels. The clinical manifestations of this disorder mirror those of familial hypercholesterolemia, including the development of tuberous and tendon xanthomata and an increased risk of premature atherosclerosis. ARH is predominantly expressed in the kidney, liver, and placenta, while expression is notably lower in tissues such as the brain, heart, muscle, colon, spleen, intestine, lung, and leukocytes, highlighting ARH′s specialized role in lipid metabolism and cellular cholesterol uptake. Anti-ARH antibody (E-10) is an essential tool for researchers investigating the mechanisms of cholesterol regulation and the pathophysiology of related disorders.

The preferred ARH antibody is ARH Antibody (F-11): sc-514106.