ARH Antibody (F-11): sc-514106

ARH Antibody (F-11) is a mouse monoclonal IgG3 kappa light chain antibody that detects the ARH protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-ARH antibody (F-11) is available as the non-conjugated form. ARH protein, also known as autosomal recessive hypercholesterolemia protein or LDLRAP1 (low density lipoprotein receptor adapter protein 1), is a crucial 308 amino acid cytoplasmic protein that plays a significant role in cholesterol metabolism. ARH protein contains one phosphotyrosine-binding domain, which is essential for functioning as an adapter protein that facilitates efficient endocytosis of low-density lipoprotein receptor (LDLR) from coated pits in polarized cells, such as hepatocytes and lymphocytes. By stabilizing interaction between LDLR and structural components of coated pits, ARH ensures proper internalization of LDLR, which is vital for maintaining cholesterol homeostasis. While ARH is predominantly expressed in kidney, liver, and placenta, ARH is found at lower levels in other tissues, including brain, heart, muscle, colon, spleen, intestine, lung, and leukocytes. Mutations in ARH gene can lead to autosomal recessive hypercholesterolemia, a condition characterized by defective internalization of LDL receptors in liver, resulting in severely elevated plasma LDL cholesterol levels and associated clinical features such as tuberous and tendon xanthomata and premature atherosclerosis.

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ARH Antibody (F-11) References:

1. Autosomal recessive hypercholesterolemia caused by mutations in a putative LDL receptor adaptor protein.  |  Garcia, CK., et al. 2001. Science. 292: 1394-8. PMID: 11326085
2. Mutation in the ARH gene and a chromosome 13q locus influence cholesterol levels in a new form of digenic-recessive familial hypercholesterolemia.  |  Al-Kateb, H., et al. 2002. Circ Res. 90: 951-8. PMID: 12016260
3. ARH is a modular adaptor protein that interacts with the LDL receptor, clathrin, and AP-2.  |  He, G., et al. 2002. J Biol Chem. 277: 44044-9. PMID: 12221107
4. Molecular mechanisms of autosomal recessive hypercholesterolemia.  |  Wilund, KR., et al. 2002. Hum Mol Genet. 11: 3019-30. PMID: 12417523
5. The autosomal recessive hypercholesterolemia (ARH) protein interfaces directly with the clathrin-coat machinery.  |  Mishra, SK., et al. 2002. Proc Natl Acad Sci U S A. 99: 16099-104. PMID: 12451172
6. Functional dissection of an AP-2 beta2 appendage-binding sequence within the autosomal recessive hypercholesterolemia protein.  |  Mishra, SK., et al. 2005. J Biol Chem. 280: 19270-80. PMID: 15728179
7. Adaptor protein ARH is recruited to the plasma membrane by low density lipoprotein (LDL) binding and modulates endocytosis of the LDL/LDL receptor complex in hepatocytes.  |  Sirinian, MI., et al. 2005. J Biol Chem. 280: 38416-23. PMID: 16129683
8. The modular adaptor protein autosomal recessive hypercholesterolemia (ARH) promotes low density lipoprotein receptor clustering into clathrin-coated pits.  |  Garuti, R., et al. 2005. J Biol Chem. 280: 40996-1004. PMID: 16179341

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ARH Antibody (F-11) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-ARH Antibody (F-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ARH Antibody (F-11) Specifications

Alternate Product Name: ARH

Product Clone Name: F-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 74-192

Epitope Target: ARH

Epitope Species: human

Product Reactivity/Detection: ARH

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for ARH Antibody (F-11): N/A