ARG1/Arginase 1 Antibody (C-2): sc-166920

Arginase 1 Antibody (C-2) is a mouse monoclonal IgG2b kappa light chain antibody engineered to detect human Arginase 1 through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-Arginase 1 antibody (C-2) targets a unique epitope mapped between amino acids 46-65 near the N-terminus of human Arginase 1, ensuring precise binding and reliable detection. Arginase 1, also called ARG1, AI, Arg-1, and PGIF, is a crucial enzyme predominantly localized in the cytoplasm of liver cells, where Arginase 1 plays a pivotal role in the urea cycle by catalyzing the hydrolysis of arginine to ornithine and urea, thereby facilitating the removal of excess nitrogen from the body. Arginase 1 exists as a homotrimeric protein containing a binuclear manganese cluster essential for catalytic activity. Arginase 1′s cellular localization in the cytoplasm ensures efficient participation in metabolic pathways that regulate amino acid metabolism and detoxify ammonia, which is vital for maintaining nitrogen balance and overall metabolic homeostasis. Understanding Arginase 1 distribution and function is important in various physiological and pathological contexts, including liver function, wound healing, immune response modulation, and cancer progression. Arginase 1′s specific localization and function make Arginase 1 a critical target for research into metabolic diseases and potential therapeutic interventions. Anti-Arginase 1 antibody (C-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatile options for researchers to study Arginase 1 in diverse experimental settings. Arginase 1 monoclonal antibody (C-2)′s high specificity and broad reactivity make this tool indispensable for scientists investigating the intricate mechanisms of arginine metabolism, enzyme regulation, and the role of Arginase 1 in health and disease.

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ARG1/Arginase 1 Antibody (C-2) References:

1. Implications of the S-shaped domain in the quaternary structure of human arginase.  |  Mora, A., et al. 2000. Biochim Biophys Acta. 1476: 181-90. PMID: 10669784
2. Structure and function of arginases.  |  Ash, DE. 2004. J Nutr. 134: 2760S-2764S; discussion 2765S-2767S. PMID: 15465781
3. Reducing arginase activity via dietary manganese deficiency enhances endothelium-dependent vasorelaxation of rat aorta.  |  Ensunsa, JL., et al. 2004. Exp Biol Med (Maywood). 229: 1143-53. PMID: 15564441
4. Hyperargininemia due to liver arginase deficiency.  |  Crombez, EA. and Cederbaum, SD. 2005. Mol Genet Metab. 84: 243-51. PMID: 15694174
5. Arginase activity concentration marking in monitoring of hepatocytes function after orthotopic liver transplantation--preliminary report.  |  Grodzicki, M., et al. 2004. Ann Transplant. 9: 54-7. PMID: 15759549
6. Remission of hepatocellular carcinoma with arginine depletion induced by systemic release of endogenous hepatic arginase due to transhepatic arterial embolisation, augmented by high-dose insulin: arginase as a potential drug candidate for hepatocellular carcinoma.  |  Cheng, PN., et al. 2005. Cancer Lett. 224: 67-80. PMID: 15911102
7. Immunological identity of the two different molecular mass constitutive subunits of liver arginase.  |  Diez, A., et al. 1994. Biol Chem Hoppe Seyler. 375: 537-41. PMID: 7811393
8. Molecular cloning of cDNA for nonhepatic mitochondrial arginase (arginase II) and comparison of its induction with nitric oxide synthase in a murine macrophage-like cell line.  |  Gotoh, T., et al. 1996. FEBS Lett. 395: 119-22. PMID: 8898077
9. Chromosomal localization of the human arginase II gene and tissue distribution of its mRNA.  |  Gotoh, T., et al. 1997. Biochem Biophys Res Commun. 233: 487-91. PMID: 9144563
10. Differential expression of arginase and iNOS in the lung in sepsis.  |  Carraway, MS., et al. 1998. Exp Lung Res. 24: 253-68. PMID: 9635249

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ARG1/Arginase 1 Antibody (C-2) has 44 citations in a variety of scientific publications.

Primary antibodies like Anti-ARG1/Arginase 1 Antibody (C-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ARG1/Arginase 1 Antibody (C-2) Specifications

Alternate Product Name: Arginase 1

Product Clone Name: C-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 46-65

Epitope Target: Arginase 1

Epitope Species: human

Product Reactivity/Detection: Arginase 1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 44 citations in various scientific publications

Available Conjugates/Forms for ARG1/Arginase 1 Antibody (C-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred ARG1/Arginase 1 antibody is ARG1/Arginase 1 Antibody (E-2): sc-271430.