Arginase 2/ARG2 Antibody (A-10) recommended for detection of Arg2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Arginase 2/ARG2 Antibody (A-10): sc-393496

Arginase 2/ARG2 Antibody (A-10) is rated 4.8 out of 5 by 5.
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Datasheets

    • Anti-Arginase 2/ARG2 Antibody (A-10) is a mouse monoclonal IgA κ Arginase 2/ARG2 antibody, cited in 5 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 304-335 near the C-terminus of Arg2 of human origin
    • recommended for detection of Arg2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and canine, bovine and porcine
    • available conjugated to agarose for IP; and to either phycoerythrin, FITC, Alexa Fluor® 488 or Alexa Fluor® 647 for IF, IHC(P) and FCM
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Arginase 2/ARG2 (A-10): sc-393496.
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Why does the image in the datasheet show a band size of 90kDa when the expected protein size is 40kDa. This antibody is suggested as a replacement for H-64 which was a rabbit monoclonal at 40kDa. So does this not detect the same protein? Thanks.

Asked by: stonology
Thank you for your question. According to the literature and our data in arginase II transfected lysates, the expected molecular weight for arginase II is approximately 40 kda. Unfortunately, we do not have any reference to support a molecular weight of 90 kda in cell lines at this time. Therefore, we are going to remove that data from our website and reevaluate the antibody in different cell lines. This mouse monoclonal antibody, arginase II (A-10): sc-393496, does detect the same arginase II protein as the now discontinued rabbit polyclonal antibody.
Answered by: Technical Support
Date published: 2017-12-07

I have two questions. First, in your data sheet, molecular weights is 40kDa. But figure of WB band says 90kDa. Is it fusion band? I wonder real band size.  Next, what secondary antibody should I use for western blotting? 

Asked by: Image
Thank you for your question. According to the literature and our data in arginase II transfected lysates, the expected molecular weight for arginase II is approximately 40 kda. Unfortunately, we do not have any reference to support a molecular weight of 90 kda in cell lines at this time. Therefore, we are going to remove that data from our website and reevaluate the antibody in different cell lines. This mouse monoclonal antibody, arginase II (A-10): sc-393496, is a mouse monoclonal IgA (kappa light chain). Ideally, we would recommend using an isotype-specific secondary antibody (anti-mouse IgA) for detection. Unfortunately, we do not currently offer this type of secondary antibody for sale.
Answered by: Technical Support
Date published: 2017-11-22

What blocking solution should I use with arginase II (A-10): sc-393496 monoclonal antibody for Western blot?

Asked by: jenni
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335) Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support 5
Date published: 2017-02-09
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Rated 4 out of 5 by from Good result I got free 10 µg sample of arginase II (A-10): sc-393496 one week ago, and prepared the HPF cell sample immidely, perfect WB result was obtained yesterday, the dilution of this antibody was 1:500, and incubated overnight at 4.
Date published: 2017-09-22
Rated 5 out of 5 by from Good performance, which really help me quite a lot two weeks ago , I applied the free sample for pre-experiment, THe results turns out to be satisfactory. I really appreciate that. and the delivery service is good, thanks
Date published: 2017-08-17
Rated 5 out of 5 by from A good choice. This antibody has some great data to support it. Immunostaining and IF is bright, clear and specific. The WB data shows strong and specific bands. This antibody is reactive with a broad range of species.
Date published: 2017-01-25
Rated 5 out of 5 by from Good signal strength in human arginase II Good signal strength in human arginase II transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-04-07
Rated 5 out of 5 by from Works well for IF applications Works well for IF applications, positive in HeLa. -SCBT QC
Date published: 2015-01-02
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