arfaptin 1 Antibody (F-3): sc-374361
- arfaptin 1 Antibody (F-3) is a mouse monoclonal IgG1 κ arfaptin 1 antibody provided at 200 µg/ml
- raised against amino acids 1-69 mapping at the N-terminus of arfaptin 1 of human origin
- arfaptin 1 Antibody (F-3) is recommended for detection of arfaptin 1 of human origin by WB, IP, IF and ELISA
- Anti-arfaptin 1 Antibody (F-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for arfaptin 1 Antibody (F-3) for WB applications. These reagents are now offered in bundles with arfaptin 1 Antibody (F-3) (see ordering information below).
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arfaptin 1 Antibody (F-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects arfaptin 1 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-arfaptin 1 antibody (F-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. arfaptin 1 plays a crucial role in regulating vesicle transport between the endoplasmic reticulum and the Golgi complex, a process essential for maintaining cellular function and communication. arfaptin 1 is recruited from the cytosol to Golgi membranes in a guanosine 5-prime-O-(3-thiotriphosphate)-dependent manner, influenced by ADP-ribosylation factors (ARFs), which are key regulators of membrane trafficking. Notably, arfaptin 1 specifically binds to nonmyristoylated GTP-bound ARF3, facilitating function in vesicle transport, while showing lower affinity for other ARFs such as ARF5 and ARF6. arfaptin 1′s interaction with ARF proteins underscores importance in cellular signaling pathways and cytoskeletal organization, making arfaptin 1 a valuable target for research in cell biology and related fields.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
arfaptin 1 Antibody (F-3) References:
- ARF6 requirement for Rac ruffling suggests a role for membrane trafficking in cortical actin rearrangements. | Radhakrishna, H., et al. 1999. J Cell Sci. 112 (Pt 6): 855-66. PMID: 10036235
- Identification of a novel Rac1-interacting protein involved in membrane ruffling. | Van Aelst, L., et al. 1996. EMBO J. 15: 3778-86. PMID: 8670882
- RAC regulation of actin polymerization and proliferation by a pathway distinct from Jun kinase. | Joneson, T., et al. 1996. Science. 274: 1374-6. PMID: 8910277
- Arfaptin 1, a putative cytosolic target protein of ADP-ribosylation factor, is recruited to Golgi membranes. | Kanoh, H., et al. 1997. J Biol Chem. 272: 5421-9. PMID: 9038142
- A role for POR1, a Rac1-interacting protein, in ARF6-mediated cytoskeletal rearrangements. | D'Souza-Schorey, C., et al. 1997. EMBO J. 16: 5445-54. PMID: 9312003
- ARF6 targets recycling vesicles to the plasma membrane: insights from an ultrastructural investigation. | D'Souza-Schorey, C., et al. 1998. J Cell Biol. 140: 603-16. PMID: 9456320
- RhoG GTPase controls a pathway that independently activates Rac1 and Cdc42Hs. | Gauthier-Rouvière, C., et al. 1998. Mol Biol Cell. 9: 1379-94. PMID: 9614181
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
arfaptin 1 Antibody (F-3) | sc-374361 | 200 µg/ml | $322.00 | |||
arfaptin 1 Antibody (F-3): m-IgG Fc BP-HRP Bundle | sc-527453 | 200 µg Ab; 10 µg BP | $361.00 | |||
arfaptin 1 Antibody (F-3): m-IgG1 BP-HRP Bundle | sc-532826 | 200 µg Ab; 20 µg BP | $361.00 | |||
arfaptin 1 Antibody (F-3) AC | sc-374361 AC | 500 µg/ml, 25% agarose | $424.00 | |||
arfaptin 1 Antibody (F-3) HRP | sc-374361 HRP | 200 µg/ml | $322.00 | |||
arfaptin 1 Antibody (F-3) FITC | sc-374361 FITC | 200 µg/ml | $336.00 | |||
arfaptin 1 Antibody (F-3) PE | sc-374361 PE | 200 µg/ml | $349.00 | |||
arfaptin 1 Antibody (F-3) Alexa Fluor® 488 | sc-374361 AF488 | 200 µg/ml | $364.00 | |||
arfaptin 1 Antibody (F-3) Alexa Fluor® 546 | sc-374361 AF546 | 200 µg/ml | $364.00 | |||
arfaptin 1 Antibody (F-3) Alexa Fluor® 594 | sc-374361 AF594 | 200 µg/ml | $364.00 | |||
arfaptin 1 Antibody (F-3) Alexa Fluor® 647 | sc-374361 AF647 | 200 µg/ml | $364.00 | |||
arfaptin 1 Antibody (F-3) Alexa Fluor® 680 | sc-374361 AF680 | 200 µg/ml | $364.00 | |||
arfaptin 1 Antibody (F-3) Alexa Fluor® 790 | sc-374361 AF790 | 200 µg/ml | $364.00 |