ARF1 Antibody (ARFS 1A9/5): sc-53168

ARF1 Antibody (ARFS 1A9/5) is a mouse monoclonal IgG2a kappa light chain antibody that detects the ARF1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. ARF1 (ARFS 1A9/5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ARF1, a member of the ADP-ribosylation factor family, plays a crucial role in cellular processes such as vesicular transport and membrane trafficking, which are vital for maintaining organelle integrity and cellular homeostasis. Located primarily at the Golgi apparatus, ARF1 is involved in the recruitment of coatomer protein complex I (COPI) to membranes, facilitating the formation of transport vesicles. This recruitment is regulated by the binding and hydrolysis of guanosine triphosphate (GTP), highlighting ARF1′s importance in the dynamic regulation of membrane traffic. ARF1′s ability to interact with specific membrane phospholipids further underscores its significance in cellular signaling and transport mechanisms. With versatile applications and critical role in cellular function, anti-ARF1 antibody (ARFS 1A9/5) serves as an essential tool for researchers studying membrane dynamics and intracellular transport pathways.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

ARF1 Antibody (ARFS 1A9/5) References:

1. Dissection of COPI and Arf1 dynamics in vivo and role in Golgi membrane transport.  |  Presley, JF., et al. 2002. Nature. 417: 187-93. PMID: 12000962
2. Structural snapshots of the mechanism and inhibition of a guanine nucleotide exchange factor.  |  Renault, L., et al. 2003. Nature. 426: 525-30. PMID: 14654833
3. Characterization of the human gene encoding ADP-ribosylation factor 1, a guanine nucleotide-binding activator of cholera toxin.  |  Lee, CM., et al. 1992. J Biol Chem. 267: 9028-34. PMID: 1577740
4. Molecular cloning, characterization, and expression of human ADP-ribosylation factors: two guanine nucleotide-dependent activators of cholera toxin.  |  Bobak, DA., et al. 1989. Proc Natl Acad Sci U S A. 86: 6101-5. PMID: 2474826
5. Molecular cloning, sequence analysis and mRNA expression of human ADP-ribosylation factor.  |  Peng, ZG., et al. 1989. Biofactors. 2: 45-9. PMID: 2535313
6. Structure of the human ADP-ribosylation factor 1 complexed with GDP.  |  Amor, JC., et al. 1994. Nature. 372: 704-8. PMID: 7990966
7. Assignment of human ADP ribosylation factor (ARF) genes ARF1 and ARF3 to chromosomes 1q42 and 12q13, respectively.  |  Hirai, M., et al. 1996. Genomics. 34: 263-5. PMID: 8661066
8. Structure of the guanine nucleotide exchange factor Sec7 domain of human arno and analysis of the interaction with ARF GTPase.  |  Mossessova, E., et al. 1998. Cell. 92: 415-23. PMID: 9476900

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ARF1 Antibody (ARFS 1A9/5) has 28 citations in a variety of scientific publications.

Primary antibodies like Anti-ARF1 Antibody (ARFS 1A9/5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ARF1 Antibody (ARFS 1A9/5) Specifications

Alternate Product Name: ARF1

Product Clone Name: ARFS 1A9/5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 174-180

Epitope Target: ARF1

Epitope Species: human

Product Reactivity/Detection: ARF1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 28 citations in various scientific publications

Available Conjugates/Forms for ARF1 Antibody (ARFS 1A9/5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin