Date published: 2025-9-5

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ARF1 抗体 (ARFS 1A9/5): sc-53168

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说明书
  • ARF1 抗体 ARFS 1A9/5 是小鼠单克隆 IgG2a κ,ARF1抗体, 在28篇文献中引用,规格为200 µg/ml
  • 免疫human物种的ARF1的氨基酸174-180
  • ARF1 抗体 (ARFS 1A9/5) 推荐用于 WB, IP, IF 和 IHC(P),检测mouse, rat 和human 来源的 ARF1
  • 抗ARF1抗体(ARFS 1A9/5)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取ARF1 (ARFS 1A9/5): sc-53168免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgGκ BP-HRP 是 ARF1 抗体 (ARFS 1A9/5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与ARF1 抗体 (ARFS 1A9/5) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

ARF1抗体(ARFS 1A9/5)是一种小鼠单克隆IgG2a kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和免疫组织化学检测小鼠、大鼠和人类来源的ARF1蛋白。ARF1 (ARFS 1A9/5) 抗体有非结合型和多种结合型可供选择,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。ARF1是ADP核糖基化因子家族的一员,在细胞过程中发挥着至关重要的作用,例如囊泡运输和膜转运,这些过程对于维持细胞器完整性和细胞内稳态至关重要。ARF1主要位于高尔基体,参与包膜蛋白复合物I(COPI)与膜的结合,促进运输囊泡的形成。这种结合受鸟苷三磷酸(GTP)的结合和水解调节,凸显了ARF1在动态调节膜运输中的重要性。ARF1能够与特定的膜磷脂相互作用,进一步凸显了其在细胞信号传导和运输机制中的重要性。抗ARF1抗体(ARFS 1A9/5)用途广泛,在细胞功能中发挥着关键作用,是研究人员研究膜动力学和细胞内运输途径的重要工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ARF1 抗体 (ARFS 1A9/5) 参考文献:

  1. 剖析 COPI 和 Arf1 在体内的动态以及在高尔基体膜运输中的作用。  |  Presley, JF., et al. 2002. Nature. 417: 187-93. PMID: 12000962
  2. 鸟嘌呤核苷酸交换因子的机制和抑制作用的结构快照。  |  Renault, L., et al. 2003. Nature. 426: 525-30. PMID: 14654833
  3. 编码 ADP-ribosylation factor 1(一种与鸟嘌呤核苷酸结合的霍乱毒素激活剂)的人类基因的特征。  |  Lee, CM., et al. 1992. J Biol Chem. 267: 9028-34. PMID: 1577740
  4. 人类 ADP 核糖基化因子的分子克隆, 表征和表达:霍乱毒素的两种鸟嘌呤核苷酸依赖性激活剂。  |  Bobak, DA., et al. 1989. Proc Natl Acad Sci U S A. 86: 6101-5. PMID: 2474826
  5. 人类 ADP-ribosylation 因子的分子克隆, 序列分析和 mRNA 表达。  |  Peng, ZG., et al. 1989. Biofactors. 2: 45-9. PMID: 2535313
  6. 人类 ADP-ribosylation 因子 1 与 GDP 复合物的结构。  |  Amor, JC., et al. 1994. Nature. 372: 704-8. PMID: 7990966
  7. 将人类 ADP 核糖基化因子(ARF)基因 ARF1 和 ARF3 分别分配到染色体 1q42 和 12q13 上。  |  Hirai, M., et al. 1996. Genomics. 34: 263-5. PMID: 8661066
  8. 人 arno 的鸟嘌呤核苷酸交换因子 Sec7 结构域及与 ARF GTPase 的相互作用分析。  |  Mossessova, E., et al. 1998. Cell. 92: 415-23. PMID: 9476900

订购信息

产品名称产品编号规格价格数量收藏夹

ARF1 抗体 (ARFS 1A9/5)

sc-53168
200 µg/ml
$316.00

ARF1 (ARFS 1A9/5): m-IgG Fc BP-HRP 套装

sc-528556
200 µg Ab; 10 µg BP
$354.00

ARF1 (ARFS 1A9/5): m-IgGκ BP-HRP 套装

sc-520956
200 µg Ab, 40 µg BP
$354.00

ARF1 抗体 (ARFS 1A9/5) AC

sc-53168 AC
500 µg/ml, 25% agarose
$416.00

ARF1 抗体 (ARFS 1A9/5) HRP

sc-53168 HRP
200 µg/ml
$316.00

ARF1 抗体 (ARFS 1A9/5) FITC

sc-53168 FITC
200 µg/ml
$330.00

ARF1 抗体 (ARFS 1A9/5) PE

sc-53168 PE
200 µg/ml
$343.00

ARF1 抗体 (ARFS 1A9/5) Alexa Fluor® 488

sc-53168 AF488
200 µg/ml
$357.00

ARF1 抗体 (ARFS 1A9/5) Alexa Fluor® 546

sc-53168 AF546
200 µg/ml
$357.00

ARF1 抗体 (ARFS 1A9/5) Alexa Fluor® 594

sc-53168 AF594
200 µg/ml
$357.00

ARF1 抗体 (ARFS 1A9/5) Alexa Fluor® 647

sc-53168 AF647
200 µg/ml
$357.00

ARF1 抗体 (ARFS 1A9/5) Alexa Fluor® 680

sc-53168 AF680
200 µg/ml
$357.00

ARF1 抗体 (ARFS 1A9/5) Alexa Fluor® 790

sc-53168 AF790
200 µg/ml
$357.00

Can ARF1 (ARFS 1A9/5): sc-53168 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: cjMara
Thank you for your question. Yes, ARF1 (ARFS 1A9/5): sc-53168 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 10
Date published: 2017-01-13

What secondary is best to use with ARF1 (ARFS 1A9/5): sc-53168?

Asked by: cjMara
Thank you for your question. While most anti-mouse IgG secondary antibodies are appropriate for use with ARF1 (ARFS 1A9/5): sc-53168, we recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 10
Date published: 2017-01-13
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Rated 5 out of 5 by from Good antibody for western blotgood antibody for western blot (1:1000, 4 degree, over-night)
Date published: 2023-10-12
Rated 5 out of 5 by from Good antibodyThis antibody is working for WB in HeLa, COS7 cells (1: 500).
Date published: 2019-05-31
Rated 4 out of 5 by from WB for mouse brain was worked.WB for mouse brain was worked. Signal intensity was normal but not strong.
Date published: 2017-12-09
Rated 5 out of 5 by from Good!We tested this hepatic cell lysate, dilution 1:500 and obtained a right single band at expected size!
Date published: 2017-08-31
Rated 5 out of 5 by from Excellent productVery nice data for western blot studies; also great IHC staining
Date published: 2017-01-23
Rated 5 out of 5 by from Good results in WBThis ARF1 antibody shows good results in western blotting.
Date published: 2017-01-14
Rated 4 out of 5 by from Good in WBVery specific signal in western blot. Not the strongest antibody but specific.
Date published: 2016-11-29
Rated 5 out of 5 by from Good specificity in WB in ZRGood specificity in WB in ZR-75-1 whole cell lysate. -SCBT QC
Date published: 2015-06-30
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ARF1 抗体 (ARFS 1A9/5) is rated 4.8 out of 5 by 14.
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