ARD1 Antibody (A-10): sc-373920
- ARD1 Antibody (A-10) is a mouse monoclonal IgG2a κ ARD1 antibody, cited in 6 publications, provided at 200 µg/ml
- raised against amino acids 1-235 representing full length ARD1 of human origin
- ARD1 Antibody (A-10) is recommended for detection of N-terminal Acetyltransferase Complex ARD1 Subunit Homolog of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-ARD1 Antibody (A-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ARD1 Antibody (A-10) for WB applications. These reagents are now offered in bundles with ARD1 Antibody (A-10) (see ordering information below).
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ARD1 Antibody (A-10) is a mouse monoclonal IgG2a kappa light chain antibody that detects ARD1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-ARD1 antibody (A-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ARD1, a member of the acetyltransferase family, plays a crucial role in cellular processes by regulating the stability of hypoxia-inducible factor-1α (HIF-1α), a key transcription factor involved in the cellular response to low oxygen levels. ARD1 is primarily located in the cytoplasm and interacts with the ribosome, NARG1, and HIF-1α, where ARD1 functions as a protein acetyltransferase. ARD1′s ability to acetylate HIF-1α influences HIF-1α degradation under normoxic conditions, thereby modulating the cellular response to hypoxia. ARD1 is cleaved by caspases during apoptosis, highlighting ARD1′s involvement in programmed cell death. ARD1 expression is downregulated in many cell lines in response to hypoxia and is expressed throughout the developing brain, underscoring ARD1′s significance in both developmental and stress-related pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ARD1 Antibody (A-10) References:
- Regulation and destabilization of HIF-1alpha by ARD1-mediated acetylation. | Jeong, JW., et al. 2002. Cell. 111: 709-20. PMID: 12464182
- An evolutionarily conserved N-terminal acetyltransferase complex associated with neuronal development. | Sugiura, N., et al. 2003. J Biol Chem. 278: 40113-20. PMID: 12888564
- E3 ubiquitin ligase activity of the trifunctional ARD1 (ADP-ribosylation factor domain protein 1). | Vichi, A., et al. 2005. Proc Natl Acad Sci U S A. 102: 1945-50. PMID: 15684077
- Analysis of ARD1 function in hypoxia response using retroviral RNA interference. | Fisher, TS., et al. 2005. J Biol Chem. 280: 17749-57. PMID: 15755738
- Differential responses of two degradation domains of HIF-1alpha to hypoxia and iron deficiency. | Lee, KH., et al. 2006. Biochimie. 88: 163-9. PMID: 16139409
- The transcriptional landscape of the mammalian genome. | Carninci, P., et al. 2005. Science. 309: 1559-63. PMID: 16141072
- Autoacetylation regulates differentially the roles of ARD1 variants in tumorigenesis. | Seo, JH., et al. 2015. Int J Oncol. 46: 99-106. PMID: 25338643
- Versatility of ARD1/NAA10-mediated protein lysine acetylation. | Vo, TTL., et al. 2018. Exp Mol Med. 50: 1-13. PMID: 30054464
- ARD1/NAA10 in hepatocellular carcinoma: pathways and clinical implications. | Lee, D., et al. 2018. Exp Mol Med. 50: 1-12. PMID: 30054466
- ARD1 contributes to IKKβ-mediated breast cancer tumorigenesis. | Zhang, Y., et al. 2018. Cell Death Dis. 9: 860. PMID: 30154412
- ARD1 stabilizes NRF2 through direct interaction and promotes colon cancer progression. | Fang, X., et al. 2023. Life Sci. 313: 121217. PMID: 36442525
- Molecular Mechanisms of ARD1 in Tumors. | Yu, C., et al. 2025. Cancer Med. 14: e70708. PMID: 40026288
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ARD1 Antibody (A-10) | sc-373920 | 200 µg/ml | $322.00 | |||
ARD1 Antibody (A-10): m-IgG Fc BP-HRP Bundle | sc-529610 | 200 µg Ab; 10 µg BP | $361.00 | |||
ARD1 Antibody (A-10): m-IgGκ BP-HRP Bundle | sc-522557 | 200 µg Ab, 40 µg BP | $361.00 | |||
ARD1 Antibody (A-10): m-IgG2a BP-HRP Bundle | sc-547339 | 200 µg Ab; 10 µg BP | $361.00 | |||
ARD1 Antibody (A-10) AC | sc-373920 AC | 500 µg/ml, 25% agarose | $424.00 | |||
ARD1 Antibody (A-10) HRP | sc-373920 HRP | 200 µg/ml | $322.00 | |||
ARD1 Antibody (A-10) FITC | sc-373920 FITC | 200 µg/ml | $336.00 | |||
ARD1 Antibody (A-10) PE | sc-373920 PE | 200 µg/ml | $349.00 | |||
ARD1 Antibody (A-10) Alexa Fluor® 488 | sc-373920 AF488 | 200 µg/ml | $364.00 | |||
ARD1 Antibody (A-10) Alexa Fluor® 546 | sc-373920 AF546 | 200 µg/ml | $364.00 | |||
ARD1 Antibody (A-10) Alexa Fluor® 594 | sc-373920 AF594 | 200 µg/ml | $364.00 | |||
ARD1 Antibody (A-10) Alexa Fluor® 647 | sc-373920 AF647 | 200 µg/ml | $364.00 | |||
ARD1 Antibody (A-10) Alexa Fluor® 680 | sc-373920 AF680 | 200 µg/ml | $364.00 | |||
ARD1 Antibody (A-10) Alexa Fluor® 790 | sc-373920 AF790 | 200 µg/ml | $364.00 |