Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Arc CRISPR/Cas9 KO Plasmid (m): sc-419184
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Arc CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Arc genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Arc Antibody (C-7): sc-17839
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Arc CRISPR/Cas9 KO Plasmid (m) | sc-419184 | 20 µg | $397.00 |
Overview
Arc (activity-regulated cytoskeleton-associated protein) is an immediate early gene induced by neuronal activity and is essential for synaptic plasticity underlying learning and memory in mouse. Arc regulates endocytic trafficking of AMPA-type glutamate receptors and coordinates actin cytoskeleton remodeling, thereby tuning synaptic strength during long-term potentiation and long-term depression. It also participates in activity-dependent gene expression programs and homeostatic synaptic scaling that stabilize neuronal network excitability. Dysregulated Arc signaling has been linked to neurodevelopmental and neuropsychiatric phenotypes and is frequently studied in the context of cognitive impairment and seizure-related network alterations.
Arc CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Arc gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Arc together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Arc open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Arc protein expression.
This CRISPR knockout system enables efficient generation of Arc-deficient cell models for investigation of Arc signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Arc exon(s) critical for Arc function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Arc genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Arc CRISPR/Cas9 KO Plasmid (m) and Arc CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Arc locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Arc HDR Plasmid (m) and Arc HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Arc homology arms to support homology-directed repair at defined Arc target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.