ARC Antibody (A-2): sc-390949

ARC Antibody (A-2) is a mouse monoclonal IgG2a antibody that detects ARC in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). ARC, also known as apoptosis repressor with CARD domain or nucleolar protein 3 (NOL3, NOP, NOP30), plays a crucial role in regulating apoptosis by inhibiting caspases, specifically caspase-2 and caspase-8, which are key mediators of programmed cell death. Anti-ARC antibody (A-2) recognizes a protein that prevents apoptosis, which is vital for cellular survival, particularly in tissues such as skeletal muscle and heart, where ARC is expressed in two transcript variants of 5.5 kb and 1.0 kb. Notably, the 1.0 kb transcript has been shown to inhibit apoptosis in a dose-dependent manner when coexpressed with caspase-8, highlighting its protective function in cellular contexts where apoptosis is triggered by external signals like CD95/FAS and tumor necrosis factor receptor-1. Furthermore, ARC is phosphorylated at Threonine 149 by casein kinase 2 (CK2), a modification that is essential for its localization to the mitochondria, thereby enhancing its role in apoptosis regulation. ARC monoclonal antibody (A-2) serves as an invaluable tool for researchers studying apoptosis and its implications in various diseases.

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ARC Antibody (A-2) References:

1. The apoptotic regulatory protein ARC (apoptosis repressor with caspase recruitment domain) prevents oxidant stress-mediated cell death by preserving mitochondrial function.  |  Neuss, M., et al. 2001. J Biol Chem. 276: 33915-22. PMID: 11438535
2. Characterization of ARC, apoptosis repressor interacting with CARD, in normal and dystrophin-deficient skeletal muscle.  |  Abmayr, S., et al. 2004. Hum Mol Genet. 13: 213-21. PMID: 14645204
3. Apoptosis repressor with caspase recruitment domain (ARC) inhibits myogenic differentiation.  |  Hunter, AL., et al. 2007. FEBS Lett. 581: 879-84. PMID: 17292893
4. ARC (apoptosis repressor with caspase recruitment domain) is a novel marker of human colon cancer.  |  Mercier, I., et al. 2008. Cell Cycle. 7: 1640-7. PMID: 18469522
5. Infarction induced myocardial apoptosis and ARC activation.  |  Ekhterae, D., et al. 2011. J Surg Res. 166: 59-67. PMID: 19815236
6. Knockdown of apoptosis repressor with caspase recruitment domain (ARC) increases the sensitivity of human glioma cell line U251MG to VM-26.  |  Wang, Q., et al. 2012. Int J Clin Exp Pathol. 5: 555-61. PMID: 22949938
7. ARC is highly expressed in nasopharyngeal carcinoma and confers X-radiation and cisplatin resistance.  |  Wu, P., et al. 2013. Oncol Rep. 30: 1807-13. PMID: 23877130
8. ARC Is a Critical Protector against Inflammatory Bowel Disease (IBD) and IBD-Associated Colorectal Tumorigenesis.  |  Wang, Q., et al. 2020. Cancer Res. 80: 4158-4171. PMID: 32816906
9. Vitamin-B12-conjugated PLGA-PEG nanoparticles incorporating miR-532-3p induce mitochondrial damage by targeting apoptosis repressor with caspase recruitment domain (ARC) on CD320-overexpressed gastric cancer.  |  Chen, Z., et al. 2021. Mater Sci Eng C Mater Biol Appl. 120: 111722. PMID: 33545873
10. ARC, an inhibitor of apoptosis expressed in skeletal muscle and heart that interacts selectively with caspases.  |  Koseki, T., et al. 1998. Proc Natl Acad Sci U S A. 95: 5156-60. PMID: 9560245

The preferred ARC antibody is ARC Antibody (F-11): sc-374177.