AR Antibody (441) recommended for detection of AR of mouse, rat and human origin by WB, IP, IF and IHC(P)

AR Antibody (441): sc-7305

AR Antibody (441) is rated 4.4 out of 5 by 49.
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Datasheets

    • Anti-AR Antibody (441) is a mouse monoclonal IgG1 κ AR antibody, cited in 476 publications, provided at 200 µg/ml
    • raised against amino acids 299-315 of AR of human origin
    • Anti-AR Antibody (441) is recommended for detection of AR of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • Anti-AR Antibody (441) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to either TRITC or Alexa Fluor® 405 for IF, IHC(P) and FCM
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-7305 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of AR (441): sc-7305.

I keep getting band at 150kDa. Do you have any idea as to what this is?

Asked by: hyunsikm
Thank you for your question. The expected molecular weight for androgen receptor (AR) is ~ 110 kDa and 87 kDa (PMID: 16946014). We do not have any information about a 150 kDa size for AR.
Answered by: Technical Support
Date published: 2019-07-31

Excuse me, I have used this antibody for about 3 months, I always have unexpected bands on my film.one unexpected band is really close to my target band, above 100KD. the other band is relatively far away,below 100KD.

Asked by: Amy Song
Thank you for your question. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2019-04-17

Do you have any references involving oocytes or ovarian tissue using IF with this antibody?

Asked by: Stella23
Thank you for your question. We are not aware of any specific publications citing use of this antibody for IF in these specific types of cells or tissues. Please contact our Technical Service Department for help with searching through the over 250 product citations for this antibody.
Answered by: Technical Support
Date published: 2018-12-19

E' possibile richiedere un campione di AR441 alexa fluor 488?

Asked by: pia79
Molte grazie per la sua domanda. La possibilità di provare i nostri anticorpi é limitata ai primari non coniugati. Consideri che offriamo un anno di garanzia dalla data di consegna per l´applicazione/specie raccomandata. In alternativa, le raccomandiamo di provare il primario non coniugato con la nostra proteina di legame IgG kappa (m-IgG BP) coniugata a CLF 488. Questo secondario si lega in maniera specifica solo alla catena leggera kappa IgG di topo, e permette un riconoscimento specifico dell´anticorpo primario con minimo legame non specifico. A supporto di questo offriamo le recensioni positive lasciate dai ricercatori. La pagina del prodotto é raggiungibile a questo link https://www.scbt.com/scbt/product/m-igg-kappa-bp-hrp Se ha interesse in testare un campione gratuito di 10 µg della proteina m-IgG BP-CFL 488 sc-516176, la prego di contattare il nostro servizio tecnico tramite telefono, email o live chat. Thank you for your question. We do not provide sample sizes of any conjugated primary antibodies. This antibody is available for purchase conjugated to Alexa Fluor® 488 sc-7305 AF488. Please note that we do offer a 1-year product warranty from the date of shipment and our warranty would apply for using the antibody for application/species reccomended. Alternatively, we would suggest to try using the unconjugated primary in conjunction with our mouse IgG kappa binding protein (m-IgG BP) conjugated to Alexa Fluor® 488. This secondary binds specifically to only the kappa light chain of mouse IgG, and enables specific detection of the primary antibody with minimal non-specific binding. We list a number of very positive customer reviews on our website for this product. A link to this product is provided below as a reference. https://www.scbt.com/scbt/product/m-igg-kappa-bp-hrp If you have an interest in testing a free 10 µg sample of m-IgG BP-CFL 488 sc-516176 please contact Technical Service by phone or email or by Live Chat.
Answered by: SCBT Heidelberg Support
Date published: 2018-11-27

I have had trouble getting this Ab (or any AR antibody I purchase from any company) to work. I normally do paraformaldehyde or formaldehyde fixation followed by ICC (cells) or do FFPE tissue with sodium citrate or EDTA tris antigen retrieval. Any tips?

Asked by: tnm60607
Thank you for your question. We would be happy to help you figure out how to get the antibody to work for your research. This antibody is recommended for IHC and IF and should be fine for this use. Please reach out to Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com.
Answered by: Tech Service
Date published: 2020-09-13

Hi I would like to do a pilot experiment to test the expression of AR in some mouse tissues. Could you please send me the free sample of AR antibody (FREE 10 µg sample of AR (441): sc-7305). Thanks!

Asked by: Mahita Kadmiel
Thank you for your question. We would be happy to send you a sample of this antibody, sc-7305. Please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com, and we would be happy to send you a sample.
Answered by: Tech Service
Date published: 2017-06-16

I need to use an antibody to detect the protein in samples of (other species, e.g., bovine, porcine, canine, equine, avian, non-mammalian) origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Tech Service 3
Date published: 2016-12-20

What is the epitope of this antibody?

Asked by: Dr Ninau Qelp
We have not mapped the epitope of this antibody. AR Antibody (441): sc-7305 was raised against amino acids 299-315 of AR of human origin. Corresponding sequence: KSTEDTAEYSPFKGGYT
Answered by: Tech Service 2
Date published: 2016-12-16
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Rated 5 out of 5 by from 使用起来不错 我之前拿到了试用装,条带跑出来很清晰,总体使用感觉不错,希望在后续试验接着使用,所以来买正式装。按付款吗
Date published: 2020-09-11
Rated 5 out of 5 by from Outstanding antibody!! This is by far the best antibody my lab has ever used to detect the full-length androgen receptor by western blot analysis of human prostate cancer cells. We have try over 6 other androgen receptor antibodies and have never had he same success. This antibody detects only one band migrating at about 110 kDa. Using this antibody at dilution of 1:1000 to 1:2000 in TBST + 3% BSA give a strong signal under 1.0 minute of film exposure using regular ECL.
Date published: 2020-04-13
Rated 4 out of 5 by from good to use Sc-7305 is good with WB experiment with rat sample.
Date published: 2019-08-13
Rated 1 out of 5 by from Does not work for mouse tissues I have tried this antibody and it simply does not work for mouse tissues on IHC-P. I previously used N-20 (sc-816) and it used to give a beautiful nuclear staining. It is very frustrating since N-20 is discontinued. It seems that 441 is not a proper subsitiute for N-20.
Date published: 2019-04-26
Rated 5 out of 5 by from Dependable AR antibody We were using N-terminal AR antibody (N-20) for long time and recently it has been replaced by AR 441 antibody. It works very similar to previous N-20 AR antibody. WE are very satisfied with this AR antibody
Date published: 2018-12-03
Rated 5 out of 5 by from Excellent specific signal I got a free sample of sc-7305 and I got a strong and specific band on LNCaP cells by WB.
Date published: 2018-10-31
Rated 5 out of 5 by from 该抗体性价比高 半年前申请了该产品试用装,一直么有评论,今天追加,抗体1;1000发光,曝光很快,条带清晰,效果很满意!
Date published: 2018-04-05
Rated 1 out of 5 by from It doesn't work at all I don't know if the problem is me or the antibody, but it doesn't work at all! I've tried different cell lines, different dilution, different way to develop....nothing! The only bands I can see are the marker ones
Date published: 2018-04-03
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