Aquaporin 7/AQP7 CRISPR/Cas9 KO Plasmid (m): sc-419178
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Aquaporin 7/AQP7 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Aquaporin 7/AQP7 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Aquaporin 7/AQP7 Antibody (D-12): sc-376407
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Aquaporin 7/AQP7 CRISPR/Cas9 KO Plasmid (m) | sc-419178 | 20 µg | $397.00 |
Overview
Aqp7 encodes aquaporin 7 (AQP7), an aquaglyceroporin that facilitates membrane transport of water and small neutral solutes such as glycerol. In mouse adipocytes, AQP7 helps regulate glycerol efflux during lipolysis, influencing glycerol utilization, triglyceride storage, and systemic energy balance. Its activity interfaces with metabolic signaling programs including cAMP/PKA-driven lipolytic pathways and broader lipid homeostasis networks. Altered AQP7 expression or function has been linked to phenotypes relevant to obesity, insulin resistance, and hepatic lipid accumulation, supporting its use as a mechanistic node in metabolic disease models.
Aquaporin 7/AQP7 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Aqp7 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Aqp7 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Aqp7 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Aquaporin 7/AQP7 protein expression.
This CRISPR knockout system enables efficient generation of Aqp7-deficient cell models for investigation of Aquaporin 7/AQP7 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Aqp7 exon(s) critical for Aquaporin 7/AQP7 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Aqp7 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Aquaporin 7/AQP7 CRISPR/Cas9 KO Plasmid (m) and Aquaporin 7/AQP7 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Aqp7 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Aquaporin 7/AQP7 HDR Plasmid (m) and Aquaporin 7/AQP7 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Aqp7 homology arms to support homology-directed repair at defined Aqp7 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.