Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Aquaporin 2/AQP2 CRISPR/Cas9 KO Plasmid (h): sc-400402
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Aquaporin 2/AQP2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Aquaporin 2/AQP2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Aquaporin 2/AQP2 Antibody (E13-21): sc-47710
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Aquaporin 2/AQP2 CRISPR/Cas9 KO Plasmid (h) | sc-400402 | 20 µg | $397.00 |
Overview
AQP2 encodes aquaporin-2, a vasopressin-regulated water channel that controls osmotic water reabsorption in renal collecting duct principal cells. In response to AVPR2–cAMP–PKA signaling, AQP2 undergoes phosphorylation-dependent trafficking to the apical membrane, coordinating with endocytic recycling and cytoskeletal dynamics to tune membrane water permeability. This pathway integrates hormonal control of fluid balance with epithelial polarity and membrane transport processes. Dysregulation of AQP2 expression, localization, or gating is linked to impaired urinary concentrating ability and serves as a mechanistic node in studies of water homeostasis and renal tubular dysfunction.
Aquaporin 2/AQP2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the AQP2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the AQP2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the AQP2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Aquaporin 2/AQP2 protein expression.
This CRISPR knockout system enables efficient generation of AQP2-deficient cell models for investigation of Aquaporin 2/AQP2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting AQP2 exon(s) critical for Aquaporin 2/AQP2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple AQP2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Aquaporin 2/AQP2 CRISPR/Cas9 KO Plasmid (h) and Aquaporin 2/AQP2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the AQP2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Aquaporin 2/AQP2 HDR Plasmid (h) and Aquaporin 2/AQP2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by AQP2 homology arms to support homology-directed repair at defined AQP2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.