Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Aquaporin 1/AQP1 CRISPR/Cas9 KO Plasmid (h): sc-400340
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Aquaporin 1/AQP1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Aquaporin 1/AQP1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Aquaporin 1/AQP1 Antibody (B-11): sc-25287
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Aquaporin 1/AQP1 CRISPR/Cas9 KO Plasmid (h) | sc-400340 | 20 µg | $397.00 |
Overview
AQP1 encodes aquaporin-1, a tetrameric membrane water channel that supports rapid osmotic water transport across plasma membranes and contributes to fluid homeostasis. In endothelial and epithelial contexts, AQP1 influences transcellular water permeability, cell volume regulation, and microenvironmental dynamics that intersect with ion transport and cytoskeletal remodeling. Altered AQP1 expression and localization have been associated with vascular biology, edema-related processes, and tumor-associated angiogenesis and cell motility phenotypes. As a tractable membrane channel, AQP1 is commonly studied to connect water flux with barrier function, migration, and stress responses.
Aquaporin 1/AQP1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the AQP1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the AQP1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the AQP1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Aquaporin 1/AQP1 protein expression.
This CRISPR knockout system enables efficient generation of AQP1-deficient cell models for investigation of Aquaporin 1/AQP1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting AQP1 exon(s) critical for Aquaporin 1/AQP1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple AQP1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Aquaporin 1/AQP1 CRISPR/Cas9 KO Plasmid (h) and Aquaporin 1/AQP1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the AQP1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Aquaporin 1/AQP1 HDR Plasmid (h) and Aquaporin 1/AQP1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by AQP1 homology arms to support homology-directed repair at defined AQP1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.