Aquaporin 0/AQP0 Antibody (B-11): sc-376445
- Aquaporin 0/AQP0 Antibody (B-11) is a mouse monoclonal IgG1 κ Aquaporin 0/AQP0 antibody, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 220-263 mapping within a C-terminal cytoplasmic domain of AQP0 of human origin
- Aquaporin 0/AQP0 Antibody (B-11) is recommended for detection of AQP0 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Aquaporin 0/AQP0 Antibody (B-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Aquaporin 0/AQP0 Antibody (B-11) for WB applications. These reagents are now offered in bundles with Aquaporin 0/AQP0 Antibody (B-11) (see ordering information below).
AQP0 Antibody (B-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects AQP0 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). AQP0 (B-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. AQP0 protein, also known as aquaporin 0, is the most abundant protein in the plasma membrane of lens fiber cells, where AQP0 plays a critical role not only as a water channel but also in maintaining fiber-fiber adhesion, which is essential for the structural integrity and organization of the lens. The unique structure of AQP0 includes an additional pore constriction that is not found in other aquaporins, allowing for precise regulation of water transport and potentially influencing the pore gating. Furthermore, the C-terminal domain of AQP0 is subject to extensive post-translational modifications, including truncations that occur during lens aging, which are mediated by m-calpain and other proteases. These modifications are significant as they may contribute to the development of cataracts, highlighting the importance of AQP0 in both lens physiology and pathology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Aquaporin 0/AQP0 Antibody (B-11) References:
- Disruption of lens fiber cell architecture in mice expressing a chimeric AQP0-LTR protein. | Shiels, A., et al. 2000. FASEB J. 14: 2207-12. PMID: 11053241
- Micro-domains of AQP0 in lens equatorial fibers. | Zampighi, GA., et al. 2002. Exp Eye Res. 75: 505-19. PMID: 12457863
- Water permeability of C-terminally truncated aquaporin 0 (AQP0 1-243) observed in the aging human lens. | Ball, LE., et al. 2003. Invest Ophthalmol Vis Sci. 44: 4820-8. PMID: 14578404
- Post-translational modifications of aquaporin 0 (AQP0) in the normal human lens: spatial and temporal occurrence. | Ball, LE., et al. 2004. Biochemistry. 43: 9856-65. PMID: 15274640
- Comparative simulations of aquaporin family: AQP1, AQPZ, AQP0 and GlpF. | Hashido, M., et al. 2005. FEBS Lett. 579: 5549-52. PMID: 16225876
- Lipid-protein interactions in double-layered two-dimensional AQP0 crystals. | Gonen, T., et al. 2005. Nature. 438: 633-8. PMID: 16319884
- AQP0-LTR of the Cat Fr mouse alters water permeability and calcium regulation of wild type AQP0. | Kalman, K., et al. 2006. Biochim Biophys Acta. 1758: 1094-9. PMID: 16515771
- Water transport in AQP0 aquaporin: molecular dynamics studies. | Han, BG., et al. 2006. J Mol Biol. 360: 285-96. PMID: 16756992
- Structure of functional single AQP0 channels in phospholipid membranes. | Zampighi, GA., et al. 2003. J Mol Biol. 325: 201-10. PMID: 12473462
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Aquaporin 0/AQP0 Antibody (B-11) | sc-376445 | 200 µg/ml | $322.00 | |||
Aquaporin 0/AQP0 Antibody (B-11): m-IgG Fc BP-HRP Bundle | sc-527536 | 200 µg Ab; 10 µg BP | $361.00 | |||
Aquaporin 0/AQP0 Antibody (B-11): m-IgG1 BP-HRP Bundle | sc-532909 | 200 µg Ab; 20 µg BP | $361.00 | |||
Aquaporin 0/AQP0 Antibody (B-11) AC | sc-376445 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Aquaporin 0/AQP0 Antibody (B-11) HRP | sc-376445 HRP | 200 µg/ml | $322.00 | |||
Aquaporin 0/AQP0 Antibody (B-11) FITC | sc-376445 FITC | 200 µg/ml | $336.00 | |||
Aquaporin 0/AQP0 Antibody (B-11) PE | sc-376445 PE | 200 µg/ml | $349.00 | |||
Aquaporin 0/AQP0 Antibody (B-11) Alexa Fluor® 488 | sc-376445 AF488 | 200 µg/ml | $364.00 | |||
Aquaporin 0/AQP0 Antibody (B-11) Alexa Fluor® 546 | sc-376445 AF546 | 200 µg/ml | $364.00 | |||
Aquaporin 0/AQP0 Antibody (B-11) Alexa Fluor® 594 | sc-376445 AF594 | 200 µg/ml | $364.00 | |||
Aquaporin 0/AQP0 Antibody (B-11) Alexa Fluor® 647 | sc-376445 AF647 | 200 µg/ml | $364.00 | |||
Aquaporin 0/AQP0 Antibody (B-11) Alexa Fluor® 680 | sc-376445 AF680 | 200 µg/ml | $364.00 | |||
Aquaporin 0/AQP0 Antibody (B-11) Alexa Fluor® 790 | sc-376445 AF790 | 200 µg/ml | $364.00 |