APPL1 Antibody (A-1): sc-271901
- APPL1 Antibody (A-1) is a mouse monoclonal IgG1 κ APPL1 antibody, cited in 7 publications, provided at 200 µg/ml
- raised against amino acids 614-709 mapping at the C-terminus of APPL1 of human origin
- APPL1 Antibody (A-1) is recommended for detection of APPL1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-APPL1 Antibody (A-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for APPL1 Antibody (A-1) for WB applications. These reagents are now offered in bundles with APPL1 Antibody (A-1) (see ordering information below).
QUICK LINKS
APPL1 Antibody (A-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects APPL1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). APPL1 Antibody (A-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. APPL1, also known as adaptor protein containing pH domain, PTB domain, and leucine zipper motif 1, plays a crucial role in cellular signaling and trafficking, particularly in epidermal growth factor (EGF) signaling. APPL1 is essential for signal translocation from plasma membrane to nucleus, where APPL1 interacts with chromatin remodeling complexes such as NuRD/MeCP1, thereby influencing gene expression and cell proliferation. APPL1 regulates the Akt signaling pathway, vital for cell survival and metabolism, by binding to kinase domains of Akt1 and Akt2. APPL1 is predominantly expressed in heart, ovary, skeletal muscle, and pancreas, highlighting APPL1′s importance in various physiological processes. With 54% homology to APPL2, APPL1 serves as a key player in the intricate network of signaling pathways that govern cellular responses to external stimuli.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
APPL1 Antibody (A-1) References:
- APPL proteins link Rab5 to nuclear signal transduction via an endosomal compartment. | Miaczynska, M., et al. 2004. Cell. 116: 445-56. PMID: 15016378
- Human follicle-stimulating hormone (FSH) receptor interacts with the adaptor protein APPL1 in HEK 293 cells: potential involvement of the PI3K pathway in FSH signaling. | Nechamen, CA., et al. 2004. Biol Reprod. 71: 629-36. PMID: 15070827
- Regulation of the Akt kinase by interacting proteins. | Du, K. and Tsichlis, PN. 2005. Oncogene. 24: 7401-9. PMID: 16288287
- APPL1 binds to adiponectin receptors and mediates adiponectin signalling and function. | Mao, X., et al. 2006. Nat Cell Biol. 8: 516-23. PMID: 16622416
- APPL1 associates with TrkA and GIPC1 and is required for nerve growth factor-mediated signal transduction. | Lin, DC., et al. 2006. Mol Cell Biol. 26: 8928-41. PMID: 17000777
- APPL1, APPL2, Akt2 and FOXO1a interact with FSHR in a potential signaling complex. | Nechamen, CA., et al. 2007. Mol Cell Endocrinol. 260-262: 93-9. PMID: 17030088
- Genetic variation within the APPL locus is not associated with metabolic or inflammatory traits in a healthy White population. | Staiger, H., et al. 2007. Diabet Med. 24: 817-22. PMID: 17490420
- Crystal structures of the BAR-PH and PTB domains of human APPL1. | Li, J., et al. 2007. Structure. 15: 525-33. PMID: 17502098
- Structure of the APPL1 BAR-PH domain and characterization of its interaction with Rab5. | Zhu, G., et al. 2007. EMBO J. 26: 3484-93. PMID: 17581628
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
APPL1 Antibody (A-1) | sc-271901 | 200 µg/ml | $322.00 | |||
APPL1 Antibody (A-1): m-IgGκ BP-HRP Bundle | sc-522094 | 200 µg Ab, 40 µg BP | $361.00 | |||
APPL1 Antibody (A-1): m-IgG1 BP-HRP Bundle | sc-533518 | 200 µg Ab; 20 µg BP | $361.00 | |||
APPL1 Antibody (A-1) AC | sc-271901 AC | 500 µg/ml, 25% agarose | $424.00 | |||
APPL1 Antibody (A-1) HRP | sc-271901 HRP | 200 µg/ml | $322.00 | |||
APPL1 Antibody (A-1) FITC | sc-271901 FITC | 200 µg/ml | $336.00 | |||
APPL1 Antibody (A-1) PE | sc-271901 PE | 200 µg/ml | $349.00 | |||
APPL1 Antibody (A-1) Alexa Fluor® 488 | sc-271901 AF488 | 200 µg/ml | $364.00 | |||
APPL1 Antibody (A-1) Alexa Fluor® 546 | sc-271901 AF546 | 200 µg/ml | $364.00 | |||
APPL1 Antibody (A-1) Alexa Fluor® 594 | sc-271901 AF594 | 200 µg/ml | $364.00 | |||
APPL1 Antibody (A-1) Alexa Fluor® 647 | sc-271901 AF647 | 200 µg/ml | $364.00 | |||
APPL1 Antibody (A-1) Alexa Fluor® 680 | sc-271901 AF680 | 200 µg/ml | $364.00 | |||
APPL1 Antibody (A-1) Alexa Fluor® 790 | sc-271901 AF790 | 200 µg/ml | $364.00 |