APIP Antibody (D-8): sc-390721
- APIP Antibody (D-8) is a mouse monoclonal IgG1 κ APIP antibody provided at 200 µg/ml
- raised against amino acids 171-242 mapping at the C-terminus of APIP of human origin
- APIP Antibody (D-8) is recommended for detection of APIP of human origin by WB, IP, IF and ELISA
- Anti-APIP Antibody (D-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for APIP Antibody (D-8) for WB applications. These reagents are now offered in bundles with APIP Antibody (D-8) (see ordering information below).
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APIP Antibody (D-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects APIP protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-APIP antibody (D-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. APIP, or Apaf-1-interacting protein, also known as APIP2, is a crucial member of the aldolase class II family, characterized by a highly conserved C-terminal region from Caenorhabditis elegans to humans. APIP is ubiquitously expressed, with particularly high levels found in the heart, kidney, and skeletal muscle, highlighting its importance in these vital tissues. Notably, APIP undergoes alternative splicing, resulting in two isoforms; isoform 1 is the full-length 242 amino acid protein, while isoform 2 lacks the first 38 residues and features a distinct sequence for amino acids 39-53. APIP prevents muscle ischemic damage by suppressing hypoxia-induced cell death through activation of Akt and ERK 1/2 signaling pathways, which inhibit caspase-9 activation through phosphorylation, and by competing with caspase-9 for binding to the caspase recruitment domain of Apaf-1. Through these mechanisms, APIP effectively regulates cell survival, making APIP a critical protein in cellular stress responses.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
APIP Antibody (D-8) References:
- Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics. | Lai, CH., et al. 2000. Genome Res. 10: 703-13. PMID: 10810093
- Cloning of a novel Apaf-1-interacting protein: a potent suppressor of apoptosis and ischemic neuronal cell death. | Cao, G., et al. 2004. J Neurosci. 24: 6189-201. PMID: 15240811
- Induced inhibition of ischemic/hypoxic injury by APIP, a novel Apaf-1-interacting protein. | Cho, DH., et al. 2004. J Biol Chem. 279: 39942-50. PMID: 15262985
- APAF-1 signaling in human melanoma. | Anichini, A., et al. 2006. Cancer Lett. 238: 168-79. PMID: 16095810
- The apoptosome: physiological, developmental, and pathological modes of regulation. | Schafer, ZT. and Kornbluth, S. 2006. Dev Cell. 10: 549-61. PMID: 16678772
- Suppression of hypoxic cell death by APIP-induced sustained activation of AKT and ERK1/2. | Cho, DH., et al. 2007. Oncogene. 26: 2809-14. PMID: 17086211
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
APIP Antibody (D-8) | sc-390721 | 200 µg/ml | $322.00 | |||
APIP Antibody (D-8): m-IgG Fc BP-HRP Bundle | sc-530240 | 200 µg Ab; 10 µg BP | $361.00 | |||
APIP Antibody (D-8): m-IgGκ BP-HRP Bundle | sc-523651 | 200 µg Ab, 40 µg BP | $361.00 | |||
APIP Antibody (D-8): m-IgG1 BP-HRP Bundle | sc-543949 | 200 µg Ab; 20 µg BP | $361.00 | |||
APIP Antibody (D-8) AC | sc-390721 AC | 500 µg/ml, 25% agarose | $424.00 | |||
APIP Antibody (D-8) HRP | sc-390721 HRP | 200 µg/ml | $322.00 | |||
APIP Antibody (D-8) FITC | sc-390721 FITC | 200 µg/ml | $336.00 | |||
APIP Antibody (D-8) PE | sc-390721 PE | 200 µg/ml | $349.00 | |||
APIP Antibody (D-8) Alexa Fluor® 488 | sc-390721 AF488 | 200 µg/ml | $364.00 | |||
APIP Antibody (D-8) Alexa Fluor® 546 | sc-390721 AF546 | 200 µg/ml | $364.00 | |||
APIP Antibody (D-8) Alexa Fluor® 594 | sc-390721 AF594 | 200 µg/ml | $364.00 | |||
APIP Antibody (D-8) Alexa Fluor® 647 | sc-390721 AF647 | 200 µg/ml | $364.00 | |||
APIP Antibody (D-8) Alexa Fluor® 680 | sc-390721 AF680 | 200 µg/ml | $364.00 | |||
APIP Antibody (D-8) Alexa Fluor® 790 | sc-390721 AF790 | 200 µg/ml | $364.00 |