Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
APC8 CRISPR/Cas9 KO Plasmid (h): sc-406761
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- APC8 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the APC8 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: APC8 Antibody (D-7): sc-514006
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
APC8 CRISPR/Cas9 KO Plasmid (h) | sc-406761 | 20 µg | $397.00 |
Overview
CDC23 encodes APC8, a core subunit of the anaphase-promoting complex/cyclosome (APC/C), an E3 ubiquitin ligase that orchestrates mitotic progression and cell-cycle exit by targeting key regulators such as securin and cyclins for proteasomal degradation. APC8 contributes to APC/C assembly and substrate recognition, integrating checkpoint signals to ensure accurate sister chromatid separation and maintenance of genome stability. Through its role in ubiquitin-mediated proteolysis, CDC23 links to pathways governing spindle checkpoint control, mitotic timing, and orderly transitions through G2/M and anaphase. Dysregulation of APC/C components and associated cell-cycle control mechanisms is frequently implicated in proliferative disorders and aneuploidy-related phenotypes, making CDC23/APC8 a relevant target for mechanistic studies of cell division defects.
APC8 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CDC23 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CDC23 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CDC23 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish APC8 protein expression.
This CRISPR knockout system enables efficient generation of CDC23-deficient cell models for investigation of APC8 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CDC23 exon(s) critical for APC8 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CDC23 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by APC8 CRISPR/Cas9 KO Plasmid (h) and APC8 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CDC23 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by APC8 HDR Plasmid (h) and APC8 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CDC23 homology arms to support homology-directed repair at defined CDC23 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.