APC8 Antibody (D-7): sc-514006
- APC8 Antibody (D-7) is a mouse monoclonal IgG1 κ APC8 antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 1-300 mapping at the N-terminus of APC8 of human origin
- APC8 Antibody (D-7) is recommended for detection of APC8 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-APC8 Antibody (D-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for APC8 Antibody (D-7) for WB applications. This reagent is now offered in a bundle with APC8 Antibody (D-7) (see ordering information below).
APC8 Antibody (D-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects APC8 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). APC8 (D-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. APC8, also known as CDC23, plays a crucial role in the anaphase-promoting complex (APC), which is essential for proper cell cycle progression. APC8 functions as a component of APC that facilitates ubiquitination of mitotic cyclins and other regulatory proteins, promoting sister chromatid separation during metaphase to anaphase transition in mitosis. This process ensures accurate chromosome segregation, which maintains genomic stability and prevents aneuploidy, a condition often associated with cancer. APC8 contains nine tetratricopeptide repeat (TPR) units, which mediate protein-protein interactions, enabling APC to function effectively. Additionally, the APC8 gene is located on human chromosome 5q31.2, a region frequently implicated in myeloid leukemias, highlighting its potential significance in cancer research.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
APC8 Antibody (D-7) References:
- Subunits and substrates of the anaphase-promoting complex. | Peters, JM. 1999. Exp Cell Res. 248: 339-49. PMID: 10222126
- Control of mitotic transitions by the anaphase-promoting complex. | Fang, G., et al. 1999. Philos Trans R Soc Lond B Biol Sci. 354: 1583-90. PMID: 10582244
- The anaphase-promoting complex: new subunits and regulators. | Page, AM. and Hieter, P. 1999. Annu Rev Biochem. 68: 583-609. PMID: 10872461
- The cyclin-ubiquitin ligase activity of cyclosome/APC is jointly activated by protein kinases Cdk1-cyclin B and Plk. | Golan, A., et al. 2002. J Biol Chem. 277: 15552-7. PMID: 11859075
- Inhibition of APC-mediated proteolysis by the meiosis-specific protein kinase Ime2. | Bolte, M., et al. 2002. Proc Natl Acad Sci U S A. 99: 4385-90. PMID: 11917129
- A subunit of the anaphase-promoting complex is a centromere-associated protein in mammalian cells. | Jörgensen, PM., et al. 1998. Mol Cell Biol. 18: 468-76. PMID: 9418894
- The structure of the tetratricopeptide repeats of protein phosphatase 5: implications for TPR-mediated protein-protein interactions. | Das, AK., et al. 1998. EMBO J. 17: 1192-9. PMID: 9482716
- Human CDC23: cDNA cloning, mapping to 5q31, genomic structure, and evaluation as a candidate tumor suppressor gene in myeloid leukemias. | Zhao, N., et al. 1998. Genomics. 53: 184-90. PMID: 9790767
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
APC8 Antibody (D-7) | sc-514006 | 200 µg/ml | $322.00 | |||
APC8 Antibody (D-7): m-IgG Fc BP-HRP Bundle | sc-526338 | 200 µg Ab; 10 µg BP | $361.00 | |||
APC8 Antibody (D-7) AC | sc-514006 AC | 500 µg/ml, 25% agarose | $424.00 | |||
APC8 Antibody (D-7) HRP | sc-514006 HRP | 200 µg/ml | $322.00 | |||
APC8 Antibody (D-7) FITC | sc-514006 FITC | 200 µg/ml | $336.00 | |||
APC8 Antibody (D-7) PE | sc-514006 PE | 200 µg/ml | $349.00 | |||
APC8 Antibody (D-7) Alexa Fluor® 488 | sc-514006 AF488 | 200 µg/ml | $364.00 | |||
APC8 Antibody (D-7) Alexa Fluor® 546 | sc-514006 AF546 | 200 µg/ml | $364.00 | |||
APC8 Antibody (D-7) Alexa Fluor® 594 | sc-514006 AF594 | 200 µg/ml | $364.00 | |||
APC8 Antibody (D-7) Alexa Fluor® 647 | sc-514006 AF647 | 200 µg/ml | $364.00 | |||
APC8 Antibody (D-7) Alexa Fluor® 680 | sc-514006 AF680 | 200 µg/ml | $364.00 | |||
APC8 Antibody (D-7) Alexa Fluor® 790 | sc-514006 AF790 | 200 µg/ml | $364.00 |