APC4 Antibody (B-11): sc-514895
- APC4 Antibody (B-11) is a mouse monoclonal IgG1 κ APC4 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 788-809 near the C-terminus of APC4 of human origin
- APC4 Antibody (B-11) is recommended for detection of APC4 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-APC4 Antibody (B-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for APC4 Antibody (B-11) for WB applications. These reagents are now offered in bundles with APC4 Antibody (B-11) (see ordering information below).
APC4 Antibody (B-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects APC4 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). APC4 (B-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The anaphase-promoting complex (APC), which includes APC4 as one of its key components, plays a crucial role in regulating the cell cycle by facilitating the transition from metaphase to anaphase during mitosis. This complex is composed of over ten subunits, including APC1, APC2, APC4, APC5, APC7, APC8, APC10, and APC11, and is essential for the ubiquitination and subsequent degradation of mitotic cyclins and other regulatory proteins, ensuring proper chromatid separation. APC4, also known as ANAPC4, consists of 808 amino acids and is vital for the assembly and function of the APC. APC activity is tightly regulated by various factors, including CDC20, CDH1, and MAD2, which modulate cyclin-ubiquitination activity and prevent premature activation. Understanding APC4 function and regulation is important for elucidating cell division mechanisms and potential implications in cancer biology, where cell cycle dysregulation can lead to tumorigenesis.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
APC4 Antibody (B-11) References:
- Subunits and substrates of the anaphase-promoting complex. | Peters, JM. 1999. Exp Cell Res. 248: 339-49. PMID: 10222126
- Control of mitotic transitions by the anaphase-promoting complex. | Fang, G., et al. 1999. Philos Trans R Soc Lond B Biol Sci. 354: 1583-90. PMID: 10582244
- The anaphase-promoting complex: new subunits and regulators. | Page, AM. and Hieter, P. 1999. Annu Rev Biochem. 68: 583-609. PMID: 10872461
- Characterisation of the human APC1, the largest subunit of the anaphase-promoting complex. | Jörgensen, PM., et al. 2001. Gene. 262: 51-9. PMID: 11179667
- The cyclin-ubiquitin ligase activity of cyclosome/APC is jointly activated by protein kinases Cdk1-cyclin B and Plk. | Golan, A., et al. 2002. J Biol Chem. 277: 15552-7. PMID: 11859075
- Inhibition of APC-mediated proteolysis by the meiosis-specific protein kinase Ime2. | Bolte, M., et al. 2002. Proc Natl Acad Sci U S A. 99: 4385-90. PMID: 11917129
- A subunit of the anaphase-promoting complex is a centromere-associated protein in mammalian cells. | Jörgensen, PM., et al. 1998. Mol Cell Biol. 18: 468-76. PMID: 9418894
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
APC4 Antibody (B-11) | sc-514895 | 200 µg/ml | $322.00 | |||
APC4 Antibody (B-11): m-IgG Fc BP-HRP Bundle | sc-527955 | 200 µg Ab; 10 µg BP | $361.00 | |||
APC4 Antibody (B-11): m-IgG1 BP-HRP Bundle | sc-533328 | 200 µg Ab; 20 µg BP | $361.00 | |||
APC4 Antibody (B-11) AC | sc-514895 AC | 500 µg/ml, 25% agarose | $424.00 | |||
APC4 Antibody (B-11) HRP | sc-514895 HRP | 200 µg/ml | $322.00 | |||
APC4 Antibody (B-11) FITC | sc-514895 FITC | 200 µg/ml | $336.00 | |||
APC4 Antibody (B-11) PE | sc-514895 PE | 200 µg/ml | $349.00 | |||
APC4 Antibody (B-11) Alexa Fluor® 488 | sc-514895 AF488 | 200 µg/ml | $364.00 | |||
APC4 Antibody (B-11) Alexa Fluor® 546 | sc-514895 AF546 | 200 µg/ml | $364.00 | |||
APC4 Antibody (B-11) Alexa Fluor® 594 | sc-514895 AF594 | 200 µg/ml | $364.00 | |||
APC4 Antibody (B-11) Alexa Fluor® 647 | sc-514895 AF647 | 200 µg/ml | $364.00 | |||
APC4 Antibody (B-11) Alexa Fluor® 680 | sc-514895 AF680 | 200 µg/ml | $364.00 | |||
APC4 Antibody (B-11) Alexa Fluor® 790 | sc-514895 AF790 | 200 µg/ml | $364.00 | |||
APC4 (B-11) Neutralizing Peptide | sc-514895 P | 100 µg/0.5 ml | $69.00 |