AP-4 Antibody (B-3): sc-166024

AP-4 Antibody (B-3) is a mouse monoclonal IgG1 antibody that detects AP-4 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). AP-4, a member of the transcription factor family, plays a crucial role in gene regulation by specifically binding to the DNA consensus sequence 5′-CAGCTG-3′, which is vital for the transcription of various genes, including those involved in immune response and cellular growth. AP-4 (B-3) antibody recognizes a protein that interacts with promoters for immunoglobulin-κ gene families and simian virus 40, highlighting AP-4′s importance in both normal cellular functions and disease states, such as cancer. Additionally, AP-4 is characterized by its helix-loop-helix structure, which is essential for DNA-binding capability and interaction with other transcription factors, thereby influencing the expression of genes critical for cellular processes. The gene encoding AP-4 is located on human chromosome 12q24, and AP-4′s expression is ubiquitous, indicating a fundamental role in various biological pathways. With diverse applications and significant biological relevance, anti-AP-4 antibody (B-3) serves as an invaluable tool for researchers studying gene regulation and its implications in health and disease.

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AP-4 Antibody (B-3) References:

1. The overexpression of AP-4 as a prognostic indicator for gastric carcinoma.  |  Xinghua, L., et al. 2012. Med Oncol. 29: 871-7. PMID: 21336989
2. AP-4 mediates export of ATG9A from the trans-Golgi network to promote autophagosome formation.  |  Mattera, R., et al. 2017. Proc Natl Acad Sci U S A. 114: E10697-E10706. PMID: 29180427
3. Transcription factor AP-4 promotes tumorigenic capability and activates the Wnt/β-catenin pathway in hepatocellular carcinoma.  |  Song, J., et al. 2018. Theranostics. 8: 3571-3583. PMID: 30026867
4. AP-4 vesicles contribute to spatial control of autophagy via RUsc-dependent peripheral delivery of ATG9A.  |  Davies, AK., et al. 2018. Nat Commun. 9: 3958. PMID: 30262884
5. Ap4A Regulates Directional Mobility and Antigen Presentation in Dendritic Cells.  |  Shu, S., et al. 2019. iScience. 16: 524-534. PMID: 31254530
6. The role of AP-4 in cargo export from the trans-Golgi network and hereditary spastic paraplegia.  |  Mattera, R., et al. 2020. Biochem Soc Trans. 48: 1877-1888. PMID: 33084855
7. AP-4-mediated axonal transport controls endocannabinoid production in neurons.  |  Davies, AK., et al. 2022. Nat Commun. 13: 1058. PMID: 35217685
8. AP-4 regulates neuronal lysosome composition, function, and transport via regulating export of critical lysosome receptor proteins at the trans-Golgi network.  |  Majumder, P., et al. 2022. Mol Biol Cell. 33: ar102. PMID: 35976706
9. The adaptor protein chaperone AAGAB stabilizes AP-4 complex subunits.  |  Mattera, R., et al. 2022. Mol Biol Cell. 33: ar109. PMID: 35976721
10. High-content screening identifies a small molecule that restores AP-4-dependent protein trafficking in neuronal models of AP-4-associated hereditary spastic paraplegia.  |  Saffari, A., et al. 2024. Nat Commun. 15: 584. PMID: 38233389

The preferred AP-4 antibody is AP-4 Antibody (A-8): sc-377042.