AP-2α/β Antibody (A6/2/2): sc-53163

AP-2α/β Antibody (A6/2/2) is a mouse monoclonal IgG1 kappa light chain antibody that detects AP-2α/β in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). AP-2α/β (A6/2/2) antibody is available in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates. The AP-2 transcription factor family, which includes AP-2α, AP-2β, and AP-2γ, is crucial for gene regulation as these proteins bind to the DNA consensus sequence CCCCAGGC, initiating transcription of specific genes. Notably, AP-2α is involved in the regulation of ectodermal-derived tissues and has been linked to developmental disorders when mutations occur in its gene, leading to conditions such as microphthalmia and corneal clouding. Additionally, AP-2β, a splice variant of AP-2α, serves to inhibit AP-2 activity, thereby playing a regulatory role in the transcriptional activity of the complex. AP-2 proteins also form part of the OB2-1 transcription factor complex, which is known to upregulate the expression of the proto-oncogene c-ErbB-2, a gene that is overexpressed in a significant percentage of breast cancers. Understanding the function and interactions of AP-2α and AP-2β is essential for elucidating their roles in both normal development and disease processes, making anti-AP-2α/β antibody (A6/2/2) a valuable tool for researchers studying these critical transcription factors.

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AP-2α/β Antibody (A6/2/2) References:

1. The transcription factors AP-2β and AP-2α are required for survival of sympathetic progenitors and differentiated sympathetic neurons.  |  Schmidt, M., et al. 2011. Dev Biol. 355: 89-100. PMID: 21539825
2. Suv39h1 mediates AP-2α-dependent inhibition of C/EBPα expression during adipogenesis.  |  Zhang, ZC., et al. 2014. Mol Cell Biol. 34: 2330-8. PMID: 24732798
3. AP-2α inhibits hepatocellular carcinoma cell growth and migration.  |  Huang, W., et al. 2016. Int J Oncol. 48: 1125-34. PMID: 26780928
4. The miR-26a/AP-2α/Nanog signaling axis mediates stem cell self-renewal and temozolomide resistance in glioma.  |  Huang, W., et al. 2019. Theranostics. 9: 5497-5516. PMID: 31534499
5. AP-2β is required for formation of the murine trabecular meshwork and Schlemm's canal.  |  Akula, M., et al. 2020. Exp Eye Res. 195: 108042. PMID: 32353428
6. Early Embryonic Expression of AP-2α Is Critical for Cardiovascular Development.  |  Johnson, AL., et al. 2020. J Cardiovasc Dev Dis. 7: PMID: 32717817
7. AP-2α and AP-2β cooperatively function in the craniofacial surface ectoderm to regulate chromatin and gene expression dynamics during facial development.  |  Van Otterloo, E., et al. 2022. Elife. 11: PMID: 35333176
8. The regulatory role of AP-2β in monoaminergic neurotransmitter systems: insights on its signalling pathway, linked disorders and theragnostic potential.  |  Al-Sabri, MH., et al. 2022. Cell Biosci. 12: 151. PMID: 36076256
9. Macroscopic inhibition of DNA damage repair pathways by targeting AP-2α with LEI110 eradicates hepatocellular carcinoma.  |  Wang, C., et al. 2024. Commun Biol. 7: 342. PMID: 38503825
10. AP-2α decreases TMZ resistance of recurrent GBM by downregulating MGMT expression and improving DNA damage.  |  Huang, G., et al. 2024. Life Sci. 357: 123111. PMID: 39369843

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-AP-2α/β Antibody (A6/2/2) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-AP-2α/β Antibody (A6/2/2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

AP-2α/β Antibody (A6/2/2) Specifications

Alternate Product Name: TFAP2A / TFAP2B

Product Clone Name: A6/2/2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: AP-2α

Epitope Species: human

Product Reactivity/Detection: AP-2α and AP-2β

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for AP-2α/β Antibody (A6/2/2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred AP-2β antibody is AP-2β Antibody (C-6): sc-390119.