AOF1 Antibody (E-3): sc-515564
- AOF1 Antibody (E-3) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- raised against amino acids 39-253 mapping near the N-terminus of AOF1 of human origin
- recommended for detection of AOF1 of mouse, rat and human origin by WB, IP, IF and ELISA
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for AOF1 Antibody (E-3). This work is in progress.
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AOF1 Antibody (E-3) is a mouse monoclonal IgG1 antibody that detects AOF1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). AOF1, also known as KDM1B or LSD2, is an 823 amino acid nuclear protein that plays a crucial role in epigenetic regulation by demethylating Lys4 of histone H3, a modification associated with transcriptional activation. This demethylation process is vital for proper gene expression, particularly during development, as AOF1 influences chromatin structure and accessibility. AOF1 exists in three alternatively spliced isoforms and is essential for de novo DNA methylation of certain imprinted genes in oocytes, highlighting AOF1′s importance in reproductive biology. AOF1 features a single SWIRM domain, which is implicated in chromatin regulation, and contains a CW-type zinc finger that may facilitate interactions with other proteins involved in gene expression. Encoded by a gene located on human chromosome 6p22.3, AOF1′s precise regulation is critical for maintaining cellular identity and function, making anti-AOF1 antibody (E-3) an invaluable tool for researchers studying epigenetic mechanisms and gene regulation.
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AOF1 Antibody (E-3) References:
- Single nucleotide polymorphism of the human high affinity choline transporter alters transport rate. | Okuda, T., et al. 2002. J Biol Chem. 277: 45315-22. PMID: 12237312
- Tiling resolution array CGH and high density expression profiling of urothelial carcinomas delineate genomic amplicons and candidate target genes specific for advanced tumors. | Heidenblad, M., et al. 2008. BMC Med Genomics. 1: 3. PMID: 18237450
- Developmental regulation of histone H3 methylation at lysine 4 in the porcine ovary. | Seneda, MM., et al. 2008. Reproduction. 135: 829-38. PMID: 18502896
- KDM1B is a histone H3K4 demethylase required to establish maternal genomic imprints. | Ciccone, DN., et al. 2009. Nature. 461: 415-8. PMID: 19727073
- AOF1 is a histone H3K4 demethylase possessing demethylase activity-independent repression function. | Yang, Z., et al. 2010. Cell Res. 20: 276-87. PMID: 20101264
- Human LSD2/KDM1b/AOF1 regulates gene transcription by modulating intragenic H3K4me2 methylation. | Fang, R., et al. 2010. Mol Cell. 39: 222-33. PMID: 20670891
- A feed-forward circuit controlling inducible NF-κB target gene activation by promoter histone demethylation. | van Essen, D., et al. 2010. Mol Cell. 39: 750-60. PMID: 20832726
- Histone H3 lysine 4 monomethylation (H3K4me1) and H3 lysine 9 monomethylation (H3K9me1): distribution and their association in regulating gene expression under hyperglycaemic/hyperinsulinemic conditions in 3T3 cells. | Gupta, J., et al. 2012. Biochimie. 94: 2656-64. PMID: 22951486
- Structure-function analysis reveals a novel mechanism for regulation of histone demethylase LSD2/AOF1/KDM1b. | Zhang, Q., et al. 2013. Cell Res. 23: 225-41. PMID: 23266887
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
AOF1 Antibody (E-3) | sc-515564 | 200 µg/ml | $322.00 |