Ankyrin G Antibody (463): sc-12719
- Ankyrin G Antibody (463) is a mouse monoclonal IgG1 κ Ankyrin G antibody, cited in 58 publications, provided at 200 µg/ml
- raised against a synthetic peptide derived from the spectrin-binding domain of Ankyrin G human origin
- Ankyrin G Antibody (463) is recommended for detection of Ankyrin G of mouse, rat and human origin by WB, IP, IF and IHC(P); also reactive with additional species, including canine
- Anti-Ankyrin G Antibody (463) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Ankyrin G Antibody (463). This work is in progress.
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Ankyrin G Antibody (463) is a mouse monoclonal IgG1 kappa light chain antibody that detects Ankyrin G protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-Ankyrin G antibody (463) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Ankyrin G protein plays a crucial role in maintaining neuronal cell integrity by anchoring sodium channels at the nodes of Ranvier and axon initial segments, essential for proper action potential propagation in neurons. This localization ensures efficient and rapid electrical signal transmission, fundamental for normal nervous system function. Ankyrin G protein is involved in cytoskeleton organization and cell shape maintenance, further highlighting its importance in cellular architecture. Ankyrin G protein exhibits tissue-specific alternative mRNA processing, resulting in two major isoforms in nervous tissue, while smaller isoforms are found in various other tissues, including lung, kidney, spleen, liver, and heart. This expression diversity demonstrates Ankyrin G protein′s multifunctional nature and significance in both neuronal and non-neuronal contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ankyrin G Antibody (463) References:
- Physiological roles of axonal ankyrins in survival of premyelinated axons and localization of voltage-gated sodium channels. | Bennett, V. and Lambert, S. 1999. J Neurocytol. 28: 303-18. PMID: 10739573
- Expression of sodium channel SNS/PN3 and ankyrin(G) mRNAs in the trigeminal ganglion after inferior alveolar nerve injury in the rat. | Bongenhielm, U., et al. 2000. Exp Neurol. 164: 384-95. PMID: 10915577
- Ankyrins, multifunctional proteins involved in many cellular pathways. | Hryniewicz-Jankowska, A., et al. 2002. Folia Histochem Cytobiol. 40: 239-49. PMID: 12219834
- Painful neuromas: a potential role for a structural transmembrane protein, ankyrin G. | Kretschmer, T., et al. 2002. J Neurosurg. 97: 1424-31. PMID: 12507143
- Lateral membrane biogenesis in human bronchial epithelial cells requires 190-kDa ankyrin-G. | Kizhatil, K. and Bennett, V. 2004. J Biol Chem. 279: 16706-14. PMID: 14757759
- Isoform specificity among ankyrins. An amphipathic alpha-helix in the divergent regulatory domain of ankyrin-b interacts with the molecular co-chaperone Hdj1/Hsp40. | Mohler, PJ., et al. 2004. J Biol Chem. 279: 25798-804. PMID: 15075330
- Elevated ankyrin G in a plexiform neurofibroma and neuromas associated with pain. | Kretschmer, T., et al. 2004. J Clin Neurosci. 11: 886-9. PMID: 15519868
- Nav1.5 E1053K mutation causing Brugada syndrome blocks binding to ankyrin-G and expression of Nav1.5 on the surface of cardiomyocytes. | Mohler, PJ., et al. 2004. Proc Natl Acad Sci U S A. 101: 17533-8. PMID: 15579534
- Roles and mechanisms of ankyrin-G in neuropsychiatric disorders. | Yoon, S., et al. 2022. Exp Mol Med. 54: 867-877. PMID: 35794211
- Dynamic interactions between E-cadherin and Ankyrin-G mediate epithelial cell polarity maintenance. | Kong, C., et al. 2023. Nat Commun. 14: 6860. PMID: 37891324
- AnkyrinG. A new ankyrin gene with neural-specific isoforms localized at the axonal initial segment and node of Ranvier. | Kordeli, E., et al. 1995. J Biol Chem. 270: 2352-9. PMID: 7836469
- Restriction of 480/270-kD ankyrin G to axon proximal segments requires multiple ankyrin G-specific domains. | Zhang, X. and Bennett, V. 1998. J Cell Biol. 142: 1571-81. PMID: 9744885
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ankyrin G Antibody (463) | sc-12719 | 200 µg/ml | $316.00 | |||
Ankyrin G Antibody (463) AC | sc-12719 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Ankyrin G Antibody (463) HRP | sc-12719 HRP | 200 µg/ml | $316.00 | |||
Ankyrin G Antibody (463) FITC | sc-12719 FITC | 200 µg/ml | $330.00 | |||
Ankyrin G Antibody (463) PE | sc-12719 PE | 200 µg/ml | $343.00 | |||
Ankyrin G Antibody (463) Alexa Fluor® 488 | sc-12719 AF488 | 200 µg/ml | $357.00 | |||
Ankyrin G Antibody (463) Alexa Fluor® 546 | sc-12719 AF546 | 200 µg/ml | $357.00 | |||
Ankyrin G Antibody (463) Alexa Fluor® 594 | sc-12719 AF594 | 200 µg/ml | $357.00 | |||
Ankyrin G Antibody (463) Alexa Fluor® 647 | sc-12719 AF647 | 200 µg/ml | $357.00 | |||
Ankyrin G Antibody (463) Alexa Fluor® 680 | sc-12719 AF680 | 200 µg/ml | $357.00 | |||
Ankyrin G Antibody (463) Alexa Fluor® 790 | sc-12719 AF790 | 200 µg/ml | $357.00 |