ANKRD11 Antibody (E-4): sc-514916
- ANKRD11 Antibody (E-4) is a mouse monoclonal IgG2a κ ANKRD11 antibody provided at 200 µg/ml
- raised against amino acids 1545-1778 mapping within an internal region of ANKRD11 of human origin
- ANKRD11 Antibody (E-4) is recommended for detection of ANKRD11 of human origin by WB, IP, IF and ELISA
- Anti-ANKRD11 Antibody (E-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for ANKRD11 Antibody (E-4) for WB applications. These reagents are now offered in bundles with ANKRD11 Antibody (E-4) (see ordering information below).
ANKRD11 Antibody (E-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects ANKRD11 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ANKRD11 (E-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ANKRD11, also known as Ankyrin repeat domain-containing protein 11, is a 2,664 amino acid nuclear protein encoded by the human gene ANKRD11. ANKRD11 plays a crucial role in gene regulation as a member of a unique family of ankyrin repeat-containing cofactors that interact with p160 nuclear receptor coactivators, enhancing target gene transcription. ANKRD11 contains four ankyrin repeats and features two intrinsic repression domains essential for transcriptional repressor function. The N-terminal repression domain (RD1) spans residues 318-611, while the C-terminal repression domain (RD2) extends from residues 2369-2663. ANKRD11 also possesses an activation domain capable of stimulating transcription in vitro, specifically within a 70-amino acid region at residues 2076-2145. This dual functionality allows ANKRD11 to modulate a balance of repression and activation signals, making ANKRD11 a significant player in gene expression regulation and cellular processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ANKRD11 Antibody (E-4) References:
- Identification of a novel family of ankyrin repeats containing cofactors for p160 nuclear receptor coactivators. | Zhang, A., et al. 2004. J Biol Chem. 279: 33799-805. PMID: 15184363
- Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. | Olsen, JV., et al. 2006. Cell. 127: 635-48. PMID: 17081983
- Subcellular localization of ankyrin repeats cofactor-1 regulates its corepressor activity. | Zhang, A., et al. 2007. J Cell Biochem. 101: 1301-15. PMID: 17286281
- Characterization of transcriptional regulatory domains of ankyrin repeat cofactor-1. | Zhang, A., et al. 2007. Biochem Biophys Res Commun. 358: 1034-40. PMID: 17521611
- An ENU-induced mutation in the Ankrd11 gene results in an osteopenia-like phenotype in the mouse mutant Yoda. | Barbaric, I., et al. 2008. Physiol Genomics. 32: 311-21. PMID: 17986521
- Characterization of ankyrin repeat-containing proteins as substrates of the asparaginyl hydroxylase factor inhibiting hypoxia-inducible transcription factor. | Linke, S., et al. 2007. Methods Enzymol. 435: 61-85. PMID: 17998049
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ANKRD11 Antibody (E-4) | sc-514916 | 200 µg/ml | $322.00 | |||
ANKRD11 Antibody (E-4): m-IgG Fc BP-HRP Bundle | sc-526403 | 200 µg Ab; 10 µg BP | $361.00 | |||
ANKRD11 Antibody (E-4): m-IgG2a BP-HRP Bundle | sc-546624 | 200 µg Ab; 10 µg BP | $361.00 | |||
ANKRD11 Antibody (E-4) AC | sc-514916 AC | 500 µg/ml, 25% agarose | $424.00 | |||
ANKRD11 Antibody (E-4) HRP | sc-514916 HRP | 200 µg/ml | $322.00 | |||
ANKRD11 Antibody (E-4) FITC | sc-514916 FITC | 200 µg/ml | $336.00 | |||
ANKRD11 Antibody (E-4) PE | sc-514916 PE | 200 µg/ml | $349.00 | |||
ANKRD11 Antibody (E-4) Alexa Fluor® 488 | sc-514916 AF488 | 200 µg/ml | $364.00 | |||
ANKRD11 Antibody (E-4) Alexa Fluor® 546 | sc-514916 AF546 | 200 µg/ml | $364.00 | |||
ANKRD11 Antibody (E-4) Alexa Fluor® 594 | sc-514916 AF594 | 200 µg/ml | $364.00 | |||
ANKRD11 Antibody (E-4) Alexa Fluor® 647 | sc-514916 AF647 | 200 µg/ml | $364.00 | |||
ANKRD11 Antibody (E-4) Alexa Fluor® 680 | sc-514916 AF680 | 200 µg/ml | $364.00 | |||
ANKRD11 Antibody (E-4) Alexa Fluor® 790 | sc-514916 AF790 | 200 µg/ml | $364.00 |