Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ANKRA CRISPR/Cas9 KO Plasmid (h): sc-417372
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ANKRA CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ANKRA genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ANKRA Antibody (A26): sc-101005
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ANKRA CRISPR/Cas9 KO Plasmid (h) | sc-417372 | 20 µg | $397.00 |
Overview
ANKRA2 (ankyrin repeat family A member 2) encodes ANKRA, an ankyrin repeat–containing protein thought to support protein–protein interaction networks that influence gene regulatory and signaling processes. Ankyrin repeat proteins commonly participate in assembly of transcriptional or chromatin-associated complexes and can modulate the stability or localization of pathway components. In human cells, ANKRA2 is studied in the context of cellular homeostasis programs such as differentiation and stress-responsive regulation where scaffold-like factors help tune pathway output. Altered regulation of ankyrin repeat–containing proteins has been linked broadly to dysregulated transcriptional control and cell-state changes relevant to disease biology, motivating mechanistic studies of ANKRA2 function.
ANKRA CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ANKRA2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ANKRA2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ANKRA2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ANKRA protein expression.
This CRISPR knockout system enables efficient generation of ANKRA2-deficient cell models for investigation of ANKRA signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ANKRA2 exon(s) critical for ANKRA function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ANKRA2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ANKRA CRISPR/Cas9 KO Plasmid (h) and ANKRA CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ANKRA2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ANKRA HDR Plasmid (h) and ANKRA HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ANKRA2 homology arms to support homology-directed repair at defined ANKRA2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.