Date published: 2025-10-15

1-800-457-3801

SCBT Portrait Logo
Seach Input

ANKRA Antibody (A26): sc-101005

3.5(2)
Write a reviewAsk a question

Datasheets
  • ANKRA Antibody (A26) is a mouse monoclonal IgG1 κ ANKRA antibody provided at 100 µg/ml
  • raised against recombinant ANKRA of human origin
  • recommended for detection of ANKRA of human origin by WB, IP, IF and ELISA
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for ANKRA Antibody (A26). This work is in progress.

    QUICK LINKS

    SEE ALSO...

    ANKRA Antibody (A26) is a mouse monoclonal IgG1 kappa light chain antibody that detects ANKRA protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-ANKRA antibody (A26) is available as the non-conjugated format. ANKRA protein, also known as ANKRA2 or RFX-B-like 2, is a 313 amino acid protein that plays a crucial role in various cellular processes due to its unique structure, which includes an N-terminal helix-loop-helix domain and three ankyrin repeats. These structural features enable ANKRA protein to interact with other proteins, such as MaxiKα, megalin, and the histone deacetylases HDAC4 and HDAC5, which are involved in regulating gene expression. ANKRA protein is typically distributed throughout the cell but translocates to the nucleus in the presence of HDACs, where ANKRA protein functions as a corepressor, binding to the C-terminal sequence of AHRR and playing a significant role in transcriptional repression. ANKRA protein′s localization can be influenced by CaMK signaling, highlighting ANKRA protein′s dynamic nature and importance in cellular signaling pathways.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

    LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

    ANKRA Antibody (A26) References:

    1. Assignment of ankyrin repeat, family A (RFXANK-like) 2 (ANKRA2) to human chromosome 5q12-->q13 by radiation hybrid mapping and somatic cell hybrid PCR.  |  Rader, K., et al. 2000. Cytogenet Cell Genet. 89: 164-5. PMID: 10965114
    2. Characterization of ANKRA, a novel ankyrin repeat protein that interacts with the cytoplasmic domain of megalin.  |  Rader, K., et al. 2000. J Am Soc Nephrol. 11: 2167-2178. PMID: 11095640
    3. Identification and functional characterization of ankyrin-repeat family protein ANKRA as a protein interacting with BKCa channel.  |  Lim, HH. and Park, CS. 2005. Mol Biol Cell. 16: 1013-25. PMID: 15616191
    4. Evolutionary conservation and characterization of the bare lymphocyte syndrome transcription factor RFX-B and its paralogue ANKRA2.  |  Long, AB. and Boss, JM. 2005. Immunogenetics. 56: 788-97. PMID: 15655668
    5. Identification of the ankyrin repeat proteins ANKRA and RFXANK as novel partners of class IIa histone deacetylases.  |  Wang, AH., et al. 2005. J Biol Chem. 280: 29117-27. PMID: 15964851
    6. New functions of the major histocompatibility complex class II-specific transcription factor RFXANK revealed by a high-resolution mutagenesis study.  |  Krawczyk, M., et al. 2005. Mol Cell Biol. 25: 8607-18. PMID: 16166641
    7. Class II histone deacetylases confer signal responsiveness to the ankyrin-repeat proteins ANKRA2 and RFXANK.  |  McKinsey, TA., et al. 2006. Mol Biol Cell. 17: 438-47. PMID: 16236793
    8. Molecular mechanism of transcriptional repression of AhR repressor involving ANKRA2, HDAC4, and HDAC5.  |  Oshima, M., et al. 2007. Biochem Biophys Res Commun. 364: 276-82. PMID: 17949687

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    ANKRA Antibody (A26)

    sc-101005
    100 µg/ml
    $333.00

    I am using ANKRA (A26): sc-101005 for immunofluorescence, what dilution of this antibody should I use?

    Asked by: Cweed
    Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
    Answered by: Technical Support
    Date published: 2017-03-24
    • y_2025, m_10, d_14, h_6CST
    • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
    • cp_1, bvpage1
    • co_hasquestionsanswers, tq_1
    • loc_en_US, sid_101005, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
    • clientName_scbt
    • bvseo_sdk, java_sdk, bvseo-4.0.0
    • CLOUD, getContent, 103ms
    • QUESTIONS, PRODUCT
    Rated 5 out of 5 by from Specific for ANKRA in Western BlotThis antibody detects over-expressed human ANKRA in transfected lysate, sc-113454. -SCBT QC
    Date published: 2023-09-14
    • y_2025, m_10, d_14, h_6
    • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
    • cp_1, bvpage1
    • co_hasreviews, tv_1, tr_1
    • loc_en_US, sid_101005, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
    • clientName_scbt
    • bvseo_sdk, java_sdk, bvseo-4.0.0
    • CLOUD, getReviews, 8ms
    • REVIEWS, PRODUCT
    ANKRA Antibody (A26) is rated 3.5 out of 5 by 2.
    • y_2025, m_10, d_14, h_6
    • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
    • cp_1, bvpage1
    • co_hasreviews, tv_1, tr_1
    • loc_en_US, sid_101005, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
    • clientName_scbt
    • bvseo_sdk, java_sdk, bvseo-4.0.0
    • CLOUD, getAggregateRating, 101ms
    • REVIEWS, PRODUCT