Anillin Antibody (B-10): sc-271814

Anillin Antibody (B-10) is a mouse monoclonal IgG2b kappa light chain antibody that detects Anillin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-Anillin antibody (B-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Anillin, also known as scraps homolog, is an evolutionarily conserved actin-binding protein that plays a crucial role in cytokinesis, a process essential for cell division. Anillin is predominantly localized in the nucleus and is highly expressed in the central nervous system, where Anillin localizes to the cleavage furrow during cytokinesis, forming a contractile ring with the assistance of Rac GTPase. The interaction of Anillin with CD2AP is vital for concentrating Rho A and ensuring proper localization of active Myosin, which is necessary for successful cleavage furrow ingression. Notably, in Anillin knockout cells, the cleavage furrow fails to complete ingression, highlighting Anillin′s critical function in cell division. Anillin expression levels are tightly regulated throughout the cell cycle, peaking during mitosis, and before cell transition into G1, Anillin associates with E-cadherin and undergoes ubiquitination by the anaphase-promoting complex/cyclosome (APC/C), which recognizes the D-box domain at Anillin′s N-terminal region. Given Anillin′s overexpression in various tumors, Anillin may serve as a potential biomarker for cancer diagnosis and prognosis, making Anillin (B-10) antibody an invaluable tool for researchers studying cell division and cancer biology implications.

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Anillin Antibody (B-10) References:

1. Ablation of PRC1 by small interfering RNA demonstrates that cytokinetic abscission requires a central spindle bundle in mammalian cells, whereas completion of furrowing does not.  |  Mollinari, C., et al. 2005. Mol Biol Cell. 16: 1043-55. PMID: 15616196
2. Clues to CD2-associated protein involvement in cytokinesis.  |  Monzo, P., et al. 2005. Mol Biol Cell. 16: 2891-902. PMID: 15800069
3. Anillin is a substrate of anaphase-promoting complex/cyclosome (APC/C) that controls spatial contractility of myosin during late cytokinesis.  |  Zhao, WM. and Fang, G. 2005. J Biol Chem. 280: 33516-24. PMID: 16040610
4. MgcRacGAP controls the assembly of the contractile ring and the initiation of cytokinesis.  |  Zhao, WM. and Fang, G. 2005. Proc Natl Acad Sci U S A. 102: 13158-63. PMID: 16129829
5. The septin-binding protein anillin is overexpressed in diverse human tumors.  |  Hall, PA., et al. 2005. Clin Cancer Res. 11: 6780-6. PMID: 16203764
6. ANLN plays a critical role in human lung carcinogenesis through the activation of RHOA and by involvement in the phosphoinositide 3-kinase/AKT pathway.  |  Suzuki, C., et al. 2005. Cancer Res. 65: 11314-25. PMID: 16357138
7. The Drosophila phosphatidylinositol transfer protein encoded by vibrator is essential to maintain cleavage-furrow ingression in cytokinesis.  |  Gatt, MK. and Glover, DM. 2006. J Cell Sci. 119: 2225-35. PMID: 16684816
8. Anillin localization defect in cardiomyocyte binucleation.  |  Engel, FB., et al. 2006. J Mol Cell Cardiol. 41: 601-12. PMID: 16889791
9. Anillin and the septins promote asymmetric ingression of the cytokinetic furrow.  |  Maddox, AS., et al. 2007. Dev Cell. 12: 827-35. PMID: 17488632

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Anillin Antibody (B-10) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-Anillin Antibody (B-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Anillin Antibody (B-10) Specifications

Alternate Product Name: Anillin

Product Clone Name: B-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 641-940

Epitope Target: Anillin

Epitope Species: human

Product Reactivity/Detection: Anillin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for Anillin Antibody (B-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin