Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Angiotensinogen CRISPR/Cas9 KO Plasmid (m): sc-419047
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Angiotensinogen CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Angiotensinogen genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Angiotensinogen Antibody (H-12): sc-374511
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Angiotensinogen CRISPR/Cas9 KO Plasmid (m) | sc-419047 | 20 µg | $397.00 |
Overview
Mouse Agt encodes angiotensinogen, a secreted liver-derived glycoprotein that serves as the precursor of angiotensin peptides within the renin–angiotensin system (RAS). Proteolytic processing by renin and angiotensin-converting enzyme generates bioactive angiotensins that regulate vascular tone, sodium and fluid homeostasis, and endocrine signaling across kidney, adrenal, heart, and brain. Angiotensinogen-mediated RAS activity intersects with inflammatory and oxidative stress pathways and influences tissue remodeling and fibrosis through angiotensin II signaling. Dysregulated Agt expression or processing is widely used as a mechanistic entry point for studying cardiovascular and renal pathophysiology, metabolic stress responses, and organ cross-talk in mouse models.
Angiotensinogen CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Agt gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Agt together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Agt open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Angiotensinogen protein expression.
This CRISPR knockout system enables efficient generation of Agt-deficient cell models for investigation of Angiotensinogen signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Agt exon(s) critical for Angiotensinogen function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Agt genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Angiotensinogen CRISPR/Cas9 KO Plasmid (m) and Angiotensinogen CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Agt locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Angiotensinogen HDR Plasmid (m) and Angiotensinogen HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Agt homology arms to support homology-directed repair at defined Agt target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.