Angiotensinogen Antibody (H-12): sc-374511

Angiotensinogen Antibody (H-12) is a mouse monoclonal IgG2b kappa light chain antibody that detects Angiotensinogen of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Angiotensinogen antibody (H-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Angiotensinogen, produced primarily in the liver, plays a crucial role in the renin-angiotensin system, which regulates blood pressure and fluid balance. Upon stimulation by low blood pressure, the kidneys secrete renin, which cleaves angiotensinogen to produce Angiotensin I, a precursor that is further converted to Angiotensin II, a potent vasoconstrictor. Angiotensin II influences extend beyond blood pressure regulation to cellular growth and remodeling, particularly in cardiac myocytes, where Angiotensin II can trigger hypertrophic responses. Angiotensin II has been shown to activate various signaling pathways, including the JAK/Stat pathway, through interaction with the angiotensin II type 1 receptor (AT1), highlighting a role in mediating cellular responses to stress and injury. Anti-Angiotensinogen antibody (H-12) effectively detects this critical protein, making this antibody an invaluable tool for researchers studying cardiovascular health, hypertension, and related disorders.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Angiotensinogen Antibody (H-12) References:

1. Vasoconstrictor-induced protein-tyrosine phosphorylation in cultured vascular smooth muscle cells.  |  Tsuda, T., et al. 1991. FEBS Lett. 285: 44-8. PMID: 2065781
2. Direct stimulation of Jak/STAT pathway by the angiotensin II AT1 receptor.  |  Marrero, MB., et al. 1995. Nature. 375: 247-50. PMID: 7746328
3. Angiotensin II-induced protein tyrosine phosphorylation in neonatal rat cardiac fibroblasts.  |  Schorb, W., et al. 1994. J Biol Chem. 269: 19626-32. PMID: 8034731
4. Linkage of Agt and Actsk-1 to distal mouse chromosome 8 loci: a new conserved linkage.  |  Abonia, JP., et al. 1993. Mamm Genome. 4: 25-32. PMID: 8093670
5. Autocrine release of angiotensin II mediates stretch-induced hypertrophy of cardiac myocytes in vitro.  |  Sadoshima, J., et al. 1993. Cell. 75: 977-84. PMID: 8252633
6. Angiotensin II induces 3CH134, a protein-tyrosine phosphatase, in vascular smooth muscle cells.  |  Duff, JL., et al. 1993. J Biol Chem. 268: 26037-40. PMID: 8253712

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Angiotensinogen Antibody (H-12) has 11 citations in a variety of scientific publications.

Primary antibodies like Anti-Angiotensinogen Antibody (H-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Angiotensinogen Antibody (H-12) Specifications

Alternate Product Name: AGT

Product Clone Name: H-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-300

Epitope Target: Angiotensinogen

Epitope Species: human

Product Reactivity/Detection: Angiotensinogen

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 11 citations in various scientific publications

Available Conjugates/Forms for Angiotensinogen Antibody (H-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin