AMPKβ1 Antibody (1A7-E11-E9): sc-517560
- AMPKβ1 Antibody (1A7-E11-E9) is a mouse monoclonal IgG provided at 100 µg/ml
- raised against recombinant AMPKβ1 protein fragments of human origin
- recommended for detection of AMPKβ1 of mouse, rat and human origin by WB, IF and IHC(P); also reactive with additional species, including donkey
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for AMPKβ1 Antibody (1A7-E11-E9). This work is in progress.
AMPKβ1 Antibody (1A7-E11-E9) is a mouse monoclonal IgG antibody that detects AMPKβ1 in mouse, rat, and human samples through applications such as western blotting (WB), immunofluorescence (IF), and immunohistochemistry. AMPKβ1 is a crucial regulatory subunit of the 5′-AMP-activated protein kinase (AMPK) complex, which plays a vital role in cellular energy homeostasis. AMPKβ1 is primarily located in the cytoplasm and is essential for the proper functioning of AMPK, as AMPKβ1 helps to stabilize the complex and facilitate activation in response to cellular stressors that lead to ATP depletion. The localization of AMPKβ1 in the cytoplasm allows for rapid signaling responses to changes in energy status, enabling cells to adapt metabolic pathways accordingly. By promoting the phosphorylation of key enzymes involved in lipid and glucose metabolism, AMPKβ1 helps maintain energy balance and prevent metabolic disorders. The human AMPKβ1 gene encodes a 271 amino acid protein, which interacts with the catalytic α subunit and the γ subunit, forming a heterotrimeric complex essential for AMPK′s function. AMPKβ1′s ability to modulate energy expenditure and metabolic processes underscores its importance in maintaining cellular health and preventing diseases associated with energy dysregulation.
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AMPKβ1 Antibody (1A7-E11-E9) References:
- AMPK-beta1 subunit is a p53-independent stress responsive protein that inhibits tumor cell growth upon forced expression. | Li, J., et al. 2003. Carcinogenesis. 24: 827-34. PMID: 12771025
- AMPK beta1 deletion reduces appetite, preventing obesity and hepatic insulin resistance. | Dzamko, N., et al. 2010. J Biol Chem. 285: 115-22. PMID: 19892703
- Hematopoietic AMPK β1 reduces mouse adipose tissue macrophage inflammation and insulin resistance in obesity. | Galic, S., et al. 2011. J Clin Invest. 121: 4903-15. PMID: 22080866
- The outcome of renal ischemia-reperfusion injury is unchanged in AMPK-β1 deficient mice. | Mount, PF., et al. 2012. PLoS One. 7: e29887. PMID: 22253816
- Reduced expression of AMPK-β1 during tumor progression enhances the oncogenic capacity of advanced ovarian cancer. | Li, C., et al. 2014. Mol Cancer. 13: 49. PMID: 24602453
- Selective Activation of AMPK β1-Containing Isoforms Improves Kidney Function in a Rat Model of Diabetic Nephropathy. | Salatto, CT., et al. 2017. J Pharmacol Exp Ther. 361: 303-311. PMID: 28289077
- AMPK β1 reduces tumor progression and improves survival in p53 null mice. | Houde, VP., et al. 2017. Mol Oncol. 11: 1143-1155. PMID: 28544264
- FR58P1a; a new uncoupler of OXPHOS that inhibits migration in triple-negative breast cancer cells via Sirt1/AMPK/β1-integrin pathway. | Urra, FA., et al. 2018. Sci Rep. 8: 13190. PMID: 30181620
- Long-chain fatty acyl-CoA esters regulate metabolism via allosteric control of AMPK β1 isoforms. | Pinkosky, SL., et al. 2020. Nat Metab. 2: 873-881. PMID: 32719536
- WW domain-binding protein 2 overexpression prevents diet-induced liver steatosis and insulin resistance through AMPKβ1. | Zheng, Z., et al. 2021. Cell Death Dis. 12: 228. PMID: 33658485
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
AMPKβ1 Antibody (1A7-E11-E9) | sc-517560 | 100 µg/ml | $322.00 |