AMPKβ1 Antibody (Z14): sc-100357

AMPKβ1 Antibody (Z14) is a mouse monoclonal IgG2a kappa light chain antibody that detects AMPKβ1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-AMPKβ1 antibody (Z14) is available as the non-conjugated format. The AMPK (5′-AMP-activated protein kinase) complex plays a crucial role in cellular energy homeostasis, consisting of a catalytic α subunit and regulatory β and γ subunits. AMPKβ1 is primarily located in the cytoplasm and is essential for sensing cellular energy levels, which is vital for maintaining metabolic balance. When cellular energy is low, AMPKβ1 is activated by an increase in AMP and a decrease in ATP, leading to the phosphorylation of key metabolic enzymes that inhibit energy-consuming processes and stimulate energy-producing pathways. This regulatory mechanism is particularly important in conditions of metabolic stress, such as during exercise or nutrient deprivation, as AMPKβ1 helps to restore energy balance and promote cell survival. The human AMPKβ1 gene encodes a 271 amino acid protein, which interacts with the α and γ subunits to form the functional AMPK complex, thereby influencing various metabolic pathways and cellular responses.

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AMPKβ1 Antibody (Z14) References:

1. Characterization of AMP-activated protein kinase gamma-subunit isoforms and their role in AMP binding.  |  Cheung, PC., et al. 2000. Biochem J. 346 Pt 3: 659-69. PMID: 10698692
2. Salsalate (Salicylate) Uncouples Mitochondria, Improves Glucose Homeostasis, and Reduces Liver Lipids Independent of AMPK-β1.  |  Smith, BK., et al. 2016. Diabetes. 65: 3352-3361. PMID: 27554471
3. AMPK β1 activation suppresses antipsychotic-induced hyperglycemia in mice.  |  Shamshoum, H., et al. 2019. FASEB J. 33: 14010-14021. PMID: 31581839
4. Salsalate reduces atherosclerosis through AMPKβ1 in mice.  |  Day, EA., et al. 2021. Mol Metab. 53: 101321. PMID: 34425254
5. Circadian rhythm modulates endochondral bone formation via MTR1/AMPKβ1/BMAL1 signaling axis.  |  Yu, S., et al. 2022. Cell Death Differ. 29: 874-887. PMID: 35094018
6. The phosphorylation of AMPKβ1 is critical for increasing autophagy and maintaining mitochondrial homeostasis in response to fatty acids.  |  Desjardins, EM., et al. 2022. Proc Natl Acad Sci U S A. 119: e2119824119. PMID: 36409897
7. Blocking AMPK β1 myristoylation enhances AMPK activity and protects mice from high-fat diet-induced obesity and hepatic steatosis.  |  Neopane, K., et al. 2022. Cell Rep. 41: 111862. PMID: 36543129
8. Macrophage AMPK β1 activation by PF-06409577 reduces the inflammatory response, cholesterol synthesis, and atherosclerosis in mice.  |  Day, EA., et al. 2023. iScience. 26: 108269. PMID: 38026185
9. Mammalian AMP-activated protein kinase subfamily.  |  Stapleton, D., et al. 1996. J Biol Chem. 271: 611-4. PMID: 8557660
10. The AMP-activated protein kinase--fuel gauge of the mammalian cell?  |  Hardie, DG. and Carling, D. 1997. Eur J Biochem. 246: 259-73. PMID: 9208914
11. AMP-activated protein kinase isoenzyme family: subunit structure and chromosomal location.  |  Stapleton, D., et al. 1997. FEBS Lett. 409: 452-6. PMID: 9224708
12. Identification of a novel AMP-activated protein kinase beta subunit isoform that is highly expressed in skeletal muscle.  |  Thornton, C., et al. 1998. J Biol Chem. 273: 12443-50. PMID: 9575201

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-AMPKβ1 Antibody (Z14) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-AMPKβ1 Antibody (Z14) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

AMPKβ1 Antibody (Z14) Specifications

Alternate Product Name: AMPKβ1

Product Clone Name: Z14

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-271

Epitope Target: AMPKβ1

Epitope Species: human

Product Reactivity/Detection: AMPKβ1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for AMPKβ1 Antibody (Z14): N/A