AMPK alpha 1/2 Antibody (D-6): sc-74461

AMPKα1/2 Antibody (D-6) is a mouse monoclonal IgG2a kappa light chain antibody raised against amino acids 251-550 mapping at the C-terminus of AMPKα1 of human origin, exhibiting reactivity with mouse, rat, and human species. Anti-AMPKα1/2 antibody (D-6) targets the alpha subunits (α1 and α2) of AMP-activated protein kinase (AMPK), a critical energy sensor and regulator of cellular metabolism. AMPK is a heterotrimeric complex composed of a catalytic α subunit and regulatory β and γ subunits. AMPK becomes activated in response to increases in the AMP/ATP ratio within cells, indicating low energy availability. Once activated, AMPK phosphorylates various downstream targets to inhibit anabolic pathways that consume ATP, such as fatty acid and cholesterol synthesis, and to stimulate catabolic pathways that generate ATP, like glucose uptake and fatty acid oxidation. This regulation is essential for maintaining energy homeostasis and has significant implications in metabolic disorders, including diabetes, obesity, and cancer. Dysregulation of AMPK activity is associated with insulin resistance and tumor growth, making AMPK a critical focus in therapeutic research. AMPKα1/2 (D-6) antibody is suitable for applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), enzyme-linked immunosorbent assay (ELISA), and immunohistochemistry, providing a valuable tool for researchers investigating cellular energy regulation, metabolic pathways, and related diseases.

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AMPK alpha 1/2 Antibody (D-6) References:

1. AMPKα1-LDHA, a new metabolic pathway, regulating stem cell fate.  |  Theret, M., et al. 2018. Cell Cycle. 17: 403-404. PMID: 29316838
2. The Role of AMPK in the Regulation of Skeletal Muscle Size, Hypertrophy, and Regeneration.  |  Thomson, DM. 2018. Int J Mol Sci. 19: PMID: 30314396
3. Endothelial AMPKα1/PRKAA1 exacerbates inflammation in HFD-fed mice.  |  Yang, Q., et al. 2022. Br J Pharmacol. 179: 1661-1678. PMID: 34796475
4. Empagliflozin attenuates cardiac microvascular ischemia/reperfusion through activating the AMPKα1/ULK1/FUNDC1/mitophagy pathway.  |  Cai, C., et al. 2022. Redox Biol. 52: 102288. PMID: 35325804
5. AMP-activated protein kinase α1 phosphorylates PHD2 to maintain systemic iron homeostasis.  |  Wang, C., et al. 2022. Clin Transl Med. 12: e854. PMID: 35538889
6. EID3 Promotes Glioma Cell Proliferation and Survival by Inactivating AMPKα1.  |  Xiang, Y., et al. 2022. J Korean Neurosurg Soc. 65: 790-800. PMID: 36344477
7. Small extracellular vesicle targeting of hypothalamic AMPKα1 promotes weight loss in leptin receptor deficient mice.  |  Milbank, E., et al. 2023. Metabolism. 139: 155350. PMID: 36423694
8. Constitutively activated AMPKα1 protects against skeletal aging in mice by promoting bone-derived IGF-1 secretion.  |  Yang, Y., et al. 2023. Cell Prolif. 56: e13476. PMID: 37042047
9. AMPKα1-mediated ZDHHC8 phosphorylation promotes the palmitoylation of SLC7A11 to facilitate ferroptosis resistance in glioblastoma.  |  Wang, Z., et al. 2024. Cancer Lett. 584: 216619. PMID: 38211651
10. Nicorandil alleviates cardiac microvascular ferroptosis in diabetic cardiomyopathy: Role of the mitochondria-localized AMPK-Parkin-ACSL4 signaling pathway.  |  Chen, Z., et al. 2024. Pharmacol Res. 200: 107057. PMID: 38218357
11. YAP upregulates AMPKα1 to induce cancer cell senescence.  |  Zhan, Y., et al. 2024. Int J Biochem Cell Biol. 170: 106559. PMID: 38499237

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-AMPK alpha 1/2 Antibody (D-6) has 147 citations in a variety of scientific publications.

Primary antibodies like Anti-AMPK alpha 1/2 Antibody (D-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

AMPK alpha 1/2 Antibody (D-6) Specifications

Alternate Product Name: AMPKα1/2

Product Clone Name: D-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 251-550

Epitope Target: AMPKα1

Epitope Species: human

Product Reactivity/Detection: AMPKα1 and AMPKα2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 147 citations in various scientific publications

Available Conjugates/Forms for AMPK alpha 1/2 Antibody (D-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin