AMPKα1 CRISPR/CAS9 KO Plasmid (h): sc-400104. Western blot analysis of AMKα1 expression in empty vector control (A) and AMPKα1 CRISPR/CAS9 KO Plasmid transfected (B) HEK293T whole cell lysates. Blot probed with AMKα1 (D-6): sc-74461. β-actin (C4): sc-47778 used as specificity and loading control.
AMPK alpha 1 CRISPR/Cas9 KO Plasmid (h): sc-400104
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- AMPK alpha 1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the AMPK alpha 1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- AMPK alpha 1 HDR Plasmid (h) (sc-400104-HDR) is recommended for co-transfection with AMPK alpha 1 CRISPR/Cas9 KO Plasmid (h) to enable selection of successfully edited cells through HDR-mediated integration of a puromycin resistance cassette and RFP reporter gene
- AMPK alpha 1 HDR Plasmid (h) is a pool of plasmids, each containing a homology-directed repair (HDR) template corresponding to the gRNA target sites in the AMPK alpha 1 CRISPR/Cas9 KO Plasmid (h)
- Each HDR plasmid contains two ~800 bp homology arms flanking the puromycin resistance and RFP cassettes, designed to bind genomic DNA sequences surrounding the Cas9-induced double-strand break site and facilitate precise HDR-mediated integration
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: AMPK alpha 1 Antibody (H-4): sc-398861
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
AMPK alpha 1 CRISPR/Cas9 KO Plasmid (h) | sc-400104 | 20 µg | $397.00 | |||
AMPK alpha 1 HDR Plasmid (h) | sc-400104-HDR | 20 µg | $445.00 |