Amphiregulin Antibody (G-4): sc-74501

Amphiregulin Antibody (G-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects Amphiregulin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-Amphiregulin antibody (G-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Amphiregulin, a member of the epidermal growth factor (EGF) family, plays a crucial role in cellular signaling and is primarily produced by keratinocytes, where Amphiregulin functions as an autocrine growth factor. Amphiregulin is vital for normal human keratinocyte proliferation and binds to the ErbB-1 receptor, which is essential for the development of epithelial tissues in the skin, lungs, and gastrointestinal tract. Research has shown that withdrawal of Amphiregulin leads to downregulation of telomerase activity in human keratinocytes, indicating Amphiregulin′s role in regulating cellular aging processes. Using Amphiregulin monoclonal antibody (G-4) in research can provide insights into the mechanisms of epithelial development and Amphiregulin′s implications in various physiological and pathological conditions.

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Amphiregulin Antibody (G-4) References:

1. Influence of aging and cell senescence on telomerase activity in keratinocytes.  |  Matsui, M., et al. 2000. J Dermatol Sci. 22: 80-7. PMID: 10674820
2. Epiregulin, a novel member of the epidermal growth factor family, is an autocrine growth factor in normal human keratinocytes.  |  Shirakata, Y., et al. 2000. J Biol Chem. 275: 5748-53. PMID: 10681561
3. Training for physical medicine.  |  TEGNER, WS. 1954. Acta Physiother Rheumatol Belg. 9: 49-52. PMID: 13180383
4. A heparin sulfate-regulated human keratinocyte autocrine factor is similar or identical to amphiregulin.  |  Cook, PW., et al. 1991. Mol Cell Biol. 11: 2547-57. PMID: 2017164
5. Emerging functions of amphiregulin in orchestrating immunity, inflammation, and tissue repair.  |  Zaiss, DMW., et al. 2015. Immunity. 42: 216-226. PMID: 25692699
6. A Macrophage-Pericyte Axis Directs Tissue Restoration via Amphiregulin-Induced Transforming Growth Factor Beta Activation.  |  Minutti, CM., et al. 2019. Immunity. 50: 645-654.e6. PMID: 30770250
7. Th17 Cell-Derived Amphiregulin Promotes Colitis-Associated Intestinal Fibrosis Through Activation of mTOR and MEK in Intestinal Myofibroblasts.  |  Zhao, X., et al. 2023. Gastroenterology. 164: 89-102. PMID: 36113570
8. Regulation of the alveolar regenerative niche by amphiregulin-producing regulatory T cells.  |  Kaiser, KA., et al. 2023. J Exp Med. 220: PMID: 36534084
9. Role of MR1-driven signals and amphiregulin on the recruitment and repair function of MAIT cells during skin wound healing.  |  du Halgouet, A., et al. 2023. Immunity. 56: 78-92.e6. PMID: 36630919
10. Amphiregulin from regulatory T cells promotes liver fibrosis and insulin resistance in non-alcoholic steatohepatitis.  |  Savage, TM., et al. 2024. Immunity. 57: 303-318.e6. PMID: 38309273
11. Sustained amphiregulin expression in intermediate alveolar stem cells drives progressive fibrosis.  |  Zhao, R., et al. 2024. Cell Stem Cell. 31: 1344-1358.e6. PMID: 39096904
12. Activation of HER4 by heparin-binding EGF-like growth factor stimulates chemotaxis but not proliferation.  |  Elenius, K., et al. 1997. EMBO J. 16: 1268-78. PMID: 9135143

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Amphiregulin Antibody (G-4) has 29 citations in a variety of scientific publications.

Primary antibodies like Anti-Amphiregulin Antibody (G-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Amphiregulin Antibody (G-4) Specifications

Alternate Product Name: AREG

Product Clone Name: G-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-155

Epitope Target: Amphiregulin

Epitope Species: human

Product Reactivity/Detection: Amphiregulin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 29 citations in various scientific publications

Available Conjugates/Forms for Amphiregulin Antibody (G-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin