Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Amphiphysin II CRISPR/Cas9 KO Plasmid (h): sc-401263
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Amphiphysin II CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Amphiphysin II genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Amphiphysin II Antibody (2F11): sc-23918
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Amphiphysin II CRISPR/Cas9 KO Plasmid (h) | sc-401263 | 20 µg | $397.00 |
Overview
BIN1 encodes amphiphysin II, a membrane curvature–sensing BAR domain protein that coordinates clathrin-mediated endocytosis, T-tubule biogenesis, and membrane remodeling through interactions with dynamin, phosphoinositides, and the actin cytoskeleton. In human cells, BIN1 contributes to trafficking and signaling homeostasis by coupling vesicle fission and cytoskeletal organization, with isoform-specific functions in muscle and nervous system contexts. Genetic and expression changes in BIN1 have been associated with myopathies, including centronuclear myopathy, and it is also implicated in neurodegeneration-related pathways such as endosomal trafficking and amyloid-associated processes relevant to Alzheimer’s disease. These roles make amphiphysin II a useful node for studying membrane dynamics, synaptic biology, and muscle cell architecture.
Amphiphysin II CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the BIN1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the BIN1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the BIN1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Amphiphysin II protein expression.
This CRISPR knockout system enables efficient generation of BIN1-deficient cell models for investigation of Amphiphysin II signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting BIN1 exon(s) critical for Amphiphysin II function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple BIN1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Amphiphysin II CRISPR/Cas9 KO Plasmid (h) and Amphiphysin II CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the BIN1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Amphiphysin II HDR Plasmid (h) and Amphiphysin II HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by BIN1 homology arms to support homology-directed repair at defined BIN1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.