Amphiphysin II Antibody (G-10): sc-74487

Amphiphysin II Antibody (G-10) is a mouse monoclonal IgG1 antibody that detects Amphiphysin II in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Amphiphysin II is a crucial protein predominantly found in the brain, where Amphiphysin II plays a vital role in synaptic vesicle recycling, a process essential for neurotransmission. Amphiphysin II functions as a dimer and features a unique structure that includes a membrane-bending BAR domain, a central Clathrin and adaptor binding domain, and a C-terminal SH3 domain. The interaction of Amphiphysin II with the GTPase Dynamin via its SH3 domain is particularly important, as this interaction facilitates the binding of Dynamin to Clathrin-coated pits, leading to vesicle budding and efficient recycling of synaptic vesicles in neurons. Additionally, Amphiphysin II has a splice variant that is highly expressed in muscle tissue, which is involved in the formation and stabilization of the T tubule network, highlighting diverse functional roles across different tissues. Anti-Amphiphysin II antibody (G-10) is an invaluable tool for researchers studying synaptic function and membrane dynamics, providing insights into the molecular mechanisms underlying neurotransmission and muscle physiology.

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Amphiphysin II Antibody (G-10) References:

1. Bridging Integrator 1 (BIN1) Genotype Effects on Working Memory, Hippocampal Volume, and Functional Connectivity in Young Healthy Individuals.  |  Zhang, X., et al. 2015. Neuropsychopharmacology. 40: 1794-803. PMID: 25630570
2. Inverse regulation of bridging integrator 1 and BCR-ABL1 in chronic myeloid leukemia.  |  Trino, S., et al. 2016. Tumour Biol. 37: 217-25. PMID: 26194865
3. BIN1 regulates dynamic t-tubule membrane.  |  Fu, Y. and Hong, T. 2016. Biochim Biophys Acta. 1863: 1839-47. PMID: 26578114
4. Post-Myocardial Infarction T-tubules Form Enlarged Branched Structures With Dysregulation of Junctophilin-2 and Bridging Integrator 1 (BIN-1).  |  Pinali, C., et al. 2017. J Am Heart Assoc. 6: PMID: 28473402
5. Amphiphysin (BIN1) negatively regulates dynamin 2 for normal muscle maturation.  |  Cowling, BS., et al. 2017. J Clin Invest. 127: 4477-4487. PMID: 29130937
6. Bridging Integrator-1 protein loss in Alzheimer's disease promotes synaptic tau accumulation and disrupts tau release.  |  Glennon, EB., et al. 2020. Brain Commun. 2: PMID: 32500121
7. MTM1 overexpression prevents and reverts BIN1-related centronuclear myopathy.  |  Giraud, Q., et al. 2023. Brain. 146: 4158-4173. PMID: 37490306
8. BIN1K358R suppresses glial response to plaques in mouse model of Alzheimer's disease.  |  Garcia-Agudo, LF., et al. 2024. Alzheimers Dement. 20: 2922-2942. PMID: 38460121
9. The Alzheimer's disease risk gene BIN1 regulates activity-dependent gene expression in human-induced glutamatergic neurons.  |  Saha, O., et al. 2024. Mol Psychiatry. 29: 2634-2646. PMID: 38514804
10. sTREM2 Mediates the Correlation Between BIN1 Gene Polymorphism and Tau Pathology in Alzheimer's Disease.  |  Guo, F., et al. 2024. J Alzheimers Dis. 101: 693-704. PMID: 39240638

The preferred Amphiphysin II antibody is Amphiphysin II Antibody (2F11): sc-23918.