Amphiphysin II Antibody (D-3): sc-74486

Amphiphysin II Antibody (D-3) is a mouse monoclonal IgG2a antibody that detects Amphiphysin II in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Amphiphysin II plays a crucial role in the brain by facilitating synaptic vesicle recycling, a process vital for neurotransmission. Amphiphysin II is primarily located at presynaptic terminals, where anti-Amphiphysin II antibody (D-3) can detect interactions with Clathrin and Dynamin, essential components for synaptic vesicle endocytosis. Amphiphysin II at these sites ensures efficient recycling of vesicles, which is critical for maintaining synaptic function and plasticity. Additionally, Amphiphysin II forms heterodimers with Amphiphysin I, enhancing functional capabilities in membrane curvature and vesicle budding. The structural integrity of Amphiphysin II, characterized by its BAR domain, allows membrane bending, which is fundamental for Clathrin-coated pit formation. Amphiphysin II monoclonal antibody (D-3) serves as an invaluable tool for researchers studying synaptic mechanisms and molecular pathways involved in neurodegenerative diseases, as well as exploring broader implications of membrane dynamics in various cellular contexts.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Amphiphysin II Antibody (D-3) References:

1. Characterization of bridging integrator 1 (BIN1) as a potential tumor suppressor and prognostic marker in hepatocellular carcinoma.  |  Pan, K., et al. 2012. Mol Med. 18: 507-18. PMID: 22281836
2. Bridging integrator 1 (BIN1) protein expression increases in the Alzheimer's disease brain and correlates with neurofibrillary tangle pathology.  |  Holler, CJ., et al. 2014. J Alzheimers Dis. 42: 1221-7. PMID: 25024306
3. Isoproterenol Promotes Rapid Ryanodine Receptor Movement to Bridging Integrator 1 (BIN1)-Organized Dyads.  |  Fu, Y., et al. 2016. Circulation. 133: 388-97. PMID: 26733606
4. Bridging Integrator 1 (BIN1) Genotypes Mediate Alzheimer's Disease Risk by Altering Neuronal Degeneration.  |  Wang, HF., et al. 2016. J Alzheimers Dis. 52: 179-90. PMID: 27003210
5. Amphiphysin (BIN1) negatively regulates dynamin 2 for normal muscle maturation.  |  Cowling, BS., et al. 2017. J Clin Invest. 127: 4477-4487. PMID: 29130937
6. Dyadic Plasticity in Cardiomyocytes.  |  Jones, PP., et al. 2018. Front Physiol. 9: 1773. PMID: 30618792
7. Hypoxia-induced exosomal circPDK1 promotes pancreatic cancer glycolysis via c-myc activation by modulating miR-628-3p/BPTF axis and degrading BIN1.  |  Lin, J., et al. 2022. J Hematol Oncol. 15: 128. PMID: 36068586
8. BIN1 regulates actin-membrane interactions during IRSp53-dependent filopodia formation.  |  Picas, L., et al. 2024. Commun Biol. 7: 549. PMID: 38724689
9. Uncovering the BIN1-SH3 interactome underpinning centronuclear myopathy.  |  Zambo, B., et al. 2024. Elife. 13: PMID: 38995680
10. BIN1 deficiency enhances ULK3-dependent autophagic flux and reduces dendritic size in mouse hippocampal neurons.  |  Jin, Y., et al. 2025. Autophagy. 21: 223-242. PMID: 39171951

The preferred Amphiphysin II antibody is Amphiphysin II Antibody (2F11): sc-23918.