Amnionless Antibody (C-10): sc-365384
- Amnionless Antibody (C-10) is a mouse monoclonal IgG1 κ Amnionless antibody, cited in 4 publications, provided at 200 µg/ml
- raised against amino acids 20-248 mapping near the N-terminus of Amnionless of mouse origin
- Amnionless Antibody (C-10) is recommended for detection of Amnionless of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-Amnionless Antibody (C-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Amnionless Antibody (C-10) for WB and IHC(P) applications. These reagents are now offered in bundles with Amnionless Antibody (C-10) (see ordering information below).
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Amnionless Antibody (C-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects Amnionless protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-Amnionless antibody (C-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Amnionless protein, encoded by the AMN gene, plays a vital role in vitamin B12 absorption, which is essential for DNA synthesis and cellular function. Defects in the AMN gene can lead to megaloblastic anemia 1 (MGA1), a hereditary disorder characterized by selective malabsorption of vitamin B12, resulting in impaired thymidine synthase activity and disrupted DNA synthesis. Amnionless protein is primarily located in the membrane of cells in the colon, kidney, and small intestine, where AMN interacts with cubulin to facilitate vitamin B12 uptake. Shorter isoforms of Amnionless protein can be found in the thymus, testis, and peripheral blood leukocytes, highlighting diverse roles in different tissues. Understanding Amnionless protein function and localization is crucial for elucidating its importance in developmental processes and nutrient absorption, making Amnionless (C-10) antibody an invaluable tool for researchers studying these pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Amnionless Antibody (C-10) References:
- The amnionless gene, essential for mouse gastrulation, encodes a visceral-endoderm-specific protein with an extracellular cysteine-rich domain. | Kalantry, S., et al. 2001. Nat Genet. 27: 412-6. PMID: 11279523
- Amnionless, essential for mouse gastrulation, is mutated in recessive hereditary megaloblastic anemia. | Tanner, SM., et al. 2003. Nat Genet. 33: 426-9. PMID: 12590260
- Mouse amnionless, which is required for primitive streak assembly, mediates cell-surface localization and endocytic function of cubilin on visceral endoderm and kidney proximal tubules. | Strope, S., et al. 2004. Development. 131: 4787-95. PMID: 15342463
- Amnionless function is required for cubilin brush-border expression and intrinsic factor-cobalamin (vitamin B12) absorption in vivo. | He, Q., et al. 2005. Blood. 106: 1447-53. PMID: 15845892
- Imerslund-Gräsbeck syndrome (selective vitamin B(12) malabsorption with proteinuria). | Gräsbeck, R. 2006. Orphanet J Rare Dis. 1: 17. PMID: 16722557
- The amn gene product is required in extraembryonic tissues for the generation of middle primitive streak derivatives. | Tomihara-Newberger, C., et al. 1998. Dev Biol. 204: 34-54. PMID: 9851841
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Amnionless Antibody (C-10) | sc-365384 | 200 µg/ml | $322.00 | |||
Amnionless Antibody (C-10): m-IgG Fc BP-HRP Bundle | sc-529366 | 200 µg Ab; 10 µg BP | $361.00 | |||
Amnionless Antibody (C-10): m-IgGκ BP-HRP Bundle | sc-522230 | 200 µg Ab, 40 µg BP | $361.00 | |||
Amnionless Antibody (C-10) AC | sc-365384 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Amnionless Antibody (C-10) HRP | sc-365384 HRP | 200 µg/ml | $322.00 | |||
Amnionless Antibody (C-10) FITC | sc-365384 FITC | 200 µg/ml | $336.00 | |||
Amnionless Antibody (C-10) PE | sc-365384 PE | 200 µg/ml | $349.00 | |||
Amnionless Antibody (C-10) Alexa Fluor® 488 | sc-365384 AF488 | 200 µg/ml | $364.00 | |||
Amnionless Antibody (C-10) Alexa Fluor® 546 | sc-365384 AF546 | 200 µg/ml | $364.00 | |||
Amnionless Antibody (C-10) Alexa Fluor® 594 | sc-365384 AF594 | 200 µg/ml | $364.00 | |||
Amnionless Antibody (C-10) Alexa Fluor® 647 | sc-365384 AF647 | 200 µg/ml | $364.00 | |||
Amnionless Antibody (C-10) Alexa Fluor® 680 | sc-365384 AF680 | 200 µg/ml | $364.00 | |||
Amnionless Antibody (C-10) Alexa Fluor® 790 | sc-365384 AF790 | 200 µg/ml | $364.00 |