Aminopeptidase P1 Antibody (H-4): sc-514272

Aminopeptidase P1 Antibody (H-4) is a mouse monoclonal IgG2b kappa light chain antibody that detects Aminopeptidase P1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Aminopeptidase P1 (H-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Aminopeptidase P1 plays a crucial role in regulating bioactive peptides, particularly in bradykinin degradation, a peptide that influences blood pressure and inflammation. This enzyme is unique in proline specificity, cleaving N-terminal amino acids when the second residue is proline, which is vital for maintaining biological activity of various peptides. Aminopeptidase P1 exists in two forms: a membrane-bound variant that is GPI-linked and a cytosolic form, with the membrane-bound version predominantly expressed in the pancreas and other tissues. Aminopeptidase P1′s presence in these locations underscores its importance in physiological processes, including inflammatory response modulation and potential implications in cancer biology, as Aminopeptidase P1 has been identified as a receptor for breast-homing peptides, suggesting a role as a therapeutic target in breast cancer treatment and prevention.

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Aminopeptidase P1 Antibody (H-4) References:

1. Cloning, expression, and characterization of human cytosolic aminopeptidase P: a single manganese(II)-dependent enzyme.  |  Cottrell, GS., et al. 2000. Biochemistry. 39: 15121-8. PMID: 11106490
2. Molecular specialization of breast vasculature: a breast-homing phage-displayed peptide binds to aminopeptidase P in breast vasculature.  |  Essler, M. and Ruoslahti, E. 2002. Proc Natl Acad Sci U S A. 99: 2252-7. PMID: 11854520
3. Kinetic and crystallographic analysis of mutant Escherichia coli aminopeptidase P: insights into substrate recognition and the mechanism of catalysis.  |  Graham, SC., et al. 2006. Biochemistry. 45: 964-75. PMID: 16411772
4. Tyrosine 387 and arginine 404 are critical in the hydrolytic mechanism of Escherichia coli aminopeptidase P.  |  Jao, SC., et al. 2006. Biochemistry. 45: 1547-53. PMID: 16460001
5. Expression of human cationic trypsinogen with an authentic N terminus using intein-mediated splicing in aminopeptidase P deficient Escherichia coli.  |  Király, O., et al. 2006. Protein Expr Purif. 48: 104-11. PMID: 16542853
6. Alterations in the expression of intestinal enzymes in rats exposed to nickel.  |  Singla, A., et al. 2006. J Appl Toxicol. 26: 397-401. PMID: 16819762
7. Kinin-dependent hypersensitivity reactions in hemodialysis: metabolic and genetic factors.  |  Molinaro, G., et al. 2006. Kidney Int. 70: 1823-31. PMID: 17003818
8. Neuropeptide Y (NPY) cleaving enzymes: structural and functional homologues of dipeptidyl peptidase 4.  |  Frerker, N., et al. 2007. Peptides. 28: 257-68. PMID: 17223229
9. Live dynamic imaging of caveolae pumping targeted antibody rapidly and specifically across endothelium in the lung.  |  Oh, P., et al. 2007. Nat Biotechnol. 25: 327-37. PMID: 17334358
10. Mitochondrial unfolded protein response gene Clpp is required to maintain ovarian follicular reserve during aging, for oocyte competence, and development of pre-implantation embryos.  |  Wang, T., et al. 2018. Aging Cell. 17: e12784. PMID: 29851234
11. Mitochondrial Stress Response Gene Clpp Is Not Required for Granulosa Cell Function.  |  Esencan, E., et al. 2020. Antioxidants (Basel). 10: PMID: 33374937
12. Purification, characterization, and cloning of a cytosolic aspartyl aminopeptidase.  |  Wilk, S., et al. 1998. J Biol Chem. 273: 15961-70. PMID: 9632644

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Aminopeptidase P1 Antibody (H-4) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-Aminopeptidase P1 Antibody (H-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Aminopeptidase P1 Antibody (H-4) Specifications

Alternate Product Name: Aminopeptidase P1

Product Clone Name: H-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 103-246

Epitope Target: Aminopeptidase P

Epitope Species: human

Product Reactivity/Detection: Aminopeptidase P1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Aminopeptidase P1 Antibody (H-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin