Aminopeptidase P1 Antibody (B-4): sc-515836

Aminopeptidase P1 Antibody (B-4) is a mouse monoclonal IgG2a antibody that detects Aminopeptidase P1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Aminopeptidase P1 is a crucial enzyme that plays a significant role in regulating bioactive peptides, particularly in bradykinin degradation, a peptide involved in vasodilation and inflammation. This enzyme is unique in proline-specific activity, cleaving N-terminal amino acids where the second residue is proline, which is essential for maintaining various peptides′ biological activity. Aminopeptidase P1 exists in two forms: a membrane-bound variant that is glycosylphosphatidylinositol (GPI)-anchored and a cytosolic form, with the membrane-bound version predominantly expressed in the pancreas and other tissues. Aminopeptidase P1 localization suggests involvement in local peptide metabolism and signaling pathways, potentially influencing physiological processes such as blood pressure regulation and immune responses. Furthermore, Aminopeptidase P1′s role as a receptor for the breast-homing peptide highlights potential as a therapeutic target in breast cancer treatment and prevention, making this protein important in both health and disease.

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Aminopeptidase P1 Antibody (B-4) References:

1. Down-regulation of the mitochondrial matrix peptidase ClpP in muscle cells causes mitochondrial dysfunction and decreases cell proliferation.  |  Deepa, SS., et al. 2016. Free Radic Biol Med. 91: 281-92. PMID: 26721594
2. Mitochondrial unfolded protein response gene CLPP changes mitochondrial dynamics and affects mitochondrial function.  |  Wu, G., et al. 2019. PeerJ. 7: e7209. PMID: 31304066
3. Impaired Mitochondrial Stress Response due to CLPP Deletion Is Associated with Altered Mitochondrial Dynamics and Increased Apoptosis in Cumulus Cells.  |  Esencan, E., et al. 2020. Reprod Sci. 27: 621-630. PMID: 31939198
4. Myricetin protects mice against MRSA-related lethal pneumonia by targeting ClpP.  |  Jing, S., et al. 2021. Biochem Pharmacol. 192: 114753. PMID: 34474040
5. Scutellarin potentiates vancomycin against lethal pneumonia caused by methicillin-resistant Staphylococcus aureus through dual inhibition of sortase A and caseinolytic peptidase P.  |  Wang, X., et al. 2022. Biochem Pharmacol. 199: 114982. PMID: 35247333
6. Nepetin reduces virulence factors expression by targeting ClpP against MRSA-induced pneumonia infection.  |  Jing, S., et al. 2022. Virulence. 13: 578-588. PMID: 35363605
7. The activated ClpP peptidase forcefully grips a protein substrate.  |  Walker, SD. and Olivares, AO. 2022. Biophys J. 121: 3907-3916. PMID: 36045571
8. Disruption of mitochondrial unfolded protein response results in telomere shortening in mouse oocytes and somatic cells.  |  Cozzolino, M., et al. 2024. Aging (Albany NY). 16: 2047-2060. PMID: 38349865
9. Development of Small Molecular Hyper-activators of Human Caseinolytic Peptidase P (hClpP) with a [1,8]-naphthyridinone Scaffold as Novel Anti-cancer Agents.  |  Fu, Y., et al. 2024. ChemMedChem. e202400528. PMID: 39505831
10. Fluorogenic substrates for bacterial aminopeptidase P and its analogs detected in human serum and calf lung.  |  Fleminger, G., et al. 1982. Eur J Biochem. 125: 609-15. PMID: 6749499

The preferred Aminopeptidase P1 antibody is Aminopeptidase P1 Antibody (H-4): sc-514272.